EMAGE mouse gene expression spatial database

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EMAGE:17193

Agk acylglycerol kinase ( MGI:1917173)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001595. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001595_01	euxassay_001595_02	euxassay_001595_03	euxassay_001595_04

euxassay_001595_05	euxassay_001595_06	euxassay_001595_07	euxassay_001595_08	euxassay_001595_09

euxassay_001595_10	euxassay_001595_11	euxassay_001595_12	euxassay_001595_13	euxassay_001595_14

euxassay_001595_15	euxassay_001595_16	euxassay_001595_17	euxassay_001595_18	euxassay_001595_19

euxassay_001595_20	euxassay_001595_21	euxassay_001595_22	euxassay_001595_23	euxassay_001595_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17193_wholemount_strong.wlz

17193_wholemount_moderate.wlz

17193_wholemount_weak.wlz

17193_wholemount_possible.wlz

17193_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17193_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09

cervical ganglion

moderate

regional

moderate expression: see section 06 07 08 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 13

tail dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4789
Entity Detected:	Agk, acylglycerol kinase ( MGI:1917173)
Sequence:	sense strand is shown >T4789 CCACGCGTCCGACACAGGAAAGGCCGGGCGCTGAGCGGCGGGCAGCGGAGAAGATGACTGCATTCTTTAA AACGCTTCGAAATCATTGGAAGAAAACTACAGCTGGGCTCTGTTTGCTGACATGGGGAGGTCACTGGCTC TACGGGAAGCACTGTGATAACCTGCTAAGAAGAGCAGCCTGTCAAGAAGCTCAGGTGTTTGGCAACCAGC TCATTCCTCCCAATGCACAAGTGAAGAAAGCCACCGTCTTTCTCAATCCCGCAGCTTGCAAAGGCAAAGC CAGAACTCTATTTGAAAAAAATGCTGCTCCAATTTTACACCTGTCTGGCATGGATGTGACTGTTGTCAAG ACGGATTATGAGGGACAAGCCAAGAAGCTCTTGGAGTTGATGGAAAGCACTGATGTGATCATTGTTGCTG GAGGAGACGGCACACTGCAGGAGGTTGTTACTGGAGTACTGAGAAGAACAGATGAGGCTACCTTCAGTAA GATTCCCATTGGATTTATCCCGCTGGGACAGACCAGTAGTTTGAGTCACACTCTCTTTGCTGAAAGTGGA AACAAAGTCCAACATATTACAGACGCCACACTTGCCATTGTGAAAGGAGAGACTGTTCCACTTGATGTCT TGCAGATCAAGGGTGAAAAAGAACAACCTGTGTATGCAATGACTGGCCTCCGGTGGGGATCTTTCAGAGA TGCTGGCGTCAAAGTTAGCAAGTACTGGTATCTTGGTCCACTAAAAACCAAAGCAGCCCACTTTTTCAGC ACTCTTCAGGAATGGCCTCAGACTCATCAAGCCTCCATCTCTTACACGGGACCTAGAGAGAGACCTCCCA TTGAGCCTGAAGAGACTCCTCCCAGGCCGTCTCTGTATAGGAGAATATTACGCAGGCTTGCCTCATTCTG GGCACAGCCACAGGATGCCTCCTCCCGAGAGGTAAGCCCTGAGGTCTGGAAAGATGTACAACTGTCCACC ATTGAACTGTCCATCACAACCCGAAACACCCAACTTGACCTGATGAGCAAAGAGGATTTTATGAACATTT GCATTGAACCTGACACTGTCAGCAAAGGAGATTTCATAATCATAGGAAGTAAGAAGGTGAGAGACCCTGG CCTGCGTGCAGCTGGCACCGAGTGCCTCCAAGCCAGCCACTGTACTCTGGTTCTTCCTGAGGGAACTGAG GGCTCCTTCAGCATTGACAGCGAGGAGTACGAAGCCATGCCGGTGGAGGTCAAACTACTGCCCAGAAAGC TGCGGTTCTTCTGTGATCCGAGGAAGAGAGAGCAAATGCTGCCGAGCACATCCCAGTGAGCGACAGCGTG GCGGGCACTCCCAGGCTGCACTGCAAGCCACCAGTGGGACCACATGGGGGATGTGTGTGCTTCTGTCCCC AGAGGGTCACAGGATCCCAAGGGAATTTTCATGGCAAGTACCCCCAGCCCTCCTGTTTGCTTCCTAGAGC ACATACCATGTCATGCACTTGTTTGTGGTCAGTACCCTGTTAACCCTCGCTTTCCTTATGTTGAGGTAGT TGATCCCTCTTACACAGTGGTTGTCTCAGGCTGGGTCTAGAACGTTCTTGTACTCCCTGAGTTGGGAAGG GCTGGAGTTTTGTGATTGTCCCACAGTGGAGAGTGGAATCTGTAGACCAAGTTCCTGTTTCGGTTCTCTG ACCGTCCTGGGGCACCCCGGGGAGGCAGCTGCTGTCTCCGGGAGCCTCGCCCTCTTTCTGGGCTCTCTCA TGGGTGCTGCTCCTTTGTGAGCTCTCTGCTTTGGTTGGTTGGTCTCATCCGGAAAGCAAATGTGAGGTTG TTCCCTCTTTAGCCATGGCAGACGTATTGTAAAATGCTATTTTAAAACAAACTAGTGCTTCCTCATTGTT AGTTTGCATGTGTACAACTGAGGCGCATGTTGAAAACTAACTGACCTGGGACCTACCCCCAGGTTGACTT TGTAACTCAACCTGGCAGGAAGCTAGGGTTCTTCATTTTTAAAAAACTGCTTCCCCAGGTGAATCAACCA TACCGGGAGAAAGTGTTTTAAATCTACTTCATAAACTTAAATGTATTATATATATATATTTTTTAAAATC ACATATTTGTGTTGACATCTATTGTTTCCATGGGAAGTTAAACACTTGTCAGTGTACTTACTAGTATTGT GTAAATATTGTTTAAAAATAAAAACTGTTTGATCATTCTTTTAAACATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5051535 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5051535 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17192 same embryo
	EMAGE:17191 same embryo
	EMAGE:17195 same embryo
	EMAGE:17194 same embryo
	EurExpress:euxassay_001595 same experiment
	MGI:4823028 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source