EMAGE mouse gene expression spatial database

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EMAGE:17205

Pank1 pantothenate kinase 1 ( MGI:1922985)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001611. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001611_01	euxassay_001611_02	euxassay_001611_03	euxassay_001611_04

euxassay_001611_05	euxassay_001611_06	euxassay_001611_07	euxassay_001611_08	euxassay_001611_09

euxassay_001611_10	euxassay_001611_11	euxassay_001611_12	euxassay_001611_13	euxassay_001611_14

euxassay_001611_15	euxassay_001611_16	euxassay_001611_17	euxassay_001611_18	euxassay_001611_19

euxassay_001611_20	euxassay_001611_21	euxassay_001611_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17205_wholemount_strong.wlz

17205_wholemount_moderate.wlz

17205_wholemount_weak.wlz

17205_wholemount_possible.wlz

17205_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17205_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 08 09 10 11

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 14 15 weak expression: see section 04

foregut-midgut junction

moderate

regional

moderate expression: see section 11 12 13

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 18 19 20

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 20 21 22

hindgut

moderate

regional

moderate expression: see section 11 12 13

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

urethra of male

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4810
Entity Detected:	Pank1, pantothenate kinase 1 ( MGI:1922985)
Sequence:	sense strand is shown >T4810 CTGGGATGTCAGTTTGTAGGTAGAGATTACGAGTTGTTTTGTTTTGTTTTGTTTTGTTGTTGTTGTTTTT TTTCCTTATCTGAGCCTAACTCCATTCAACTGGTTACCTCTTTGTGGGTGTCTTTAATGAAGCTTGTAAA TGGCAGAAAGCAAACATTCCCGTGGTTTGGTATGGATATCGGTGGAACCCTGGTTAAGCTGGTTTACTTT GAACCGAAGGATATCACGGCAGAGGAAGAACAGGAAGAAGTGGAGAACCTGAAGAGCATCCGGAAGTATT TAACTTCTAACACTGCCTACGGCAAAACTGGGATCCGGGACGTCCATCTGGAACTGAAAAACCTGACCAT GTGTGGGCGCAAAGGGAACCTGCACTTCATCCGCTTCCCGACCTGTGCCATGCACTTGTTCATCCAGATG GGCAGCGAGAAGAACTTCTCCAGCCTCCACACCACCCTCTGTGCCACGGGAGGTGGGGCTTTCAAGTTTG AGGAGGACTTCCGAATGATTGCGGACCTGCAGCTGCATAAACTGGATGAGCTGGACTGTTTGATTCAGGG CCTTCTTTACGTTGACTCGGTTGGCTTCAATGGCAAGCCAGAATGTTACTATTTCGAAAACCCCACAAAT CCCGAGTTGTGTCAAAAAAAGCCATACTGTTTGGATAACCCGTACCCTATGTTGCTGGTTAACATGGGCT CAGGCGTCAGCATCCTGGCAGTGTACTCCAAGGACAACTACAAAAGAGTGACGGGGACCAGTCTTGGAGG TGGGACATTCCTAGGCCTATGTTGCTTGCTGACTGGTTGTGAGACCTTTGAAGAAGCTCTGGACATGGCA GCTAAAGGCGACAGCACCAATGTGGATAAGCTGGTGAAGGACATTTACGGAGGAGACTATGAGCGATTTG GCCTTCAAGGATCTGCTGTAGCATCAAGCTTTGGCAACATGATGAGCAAAGAAAAGAGAGAGTCCATCAG CAAAGAAGACCTCGCCCGGGCCACATTGGTCACCATCACCAACAACATTGGCTCCATTGCTCGGATGTGT GCACTGAATGAGAATATTGACAGAGTTGTGTTTGTTGGGAACTTTCTCAGAATCAATATGGTCTCAATGA AGTTGCTAGCATATGCCATGGACTTTTGGTCTAAAGGACAGCTGAAAGCACTGTTTTTGGAACATGAGGG TTATTTTGGAGCTGTTGGGGCCCTGTTGGAACTGTTCAAAATGACCGATACACAGTAGAGGAACAGCCCC TCGTGCGGACAGAGGACTGACGGGCCTTGCCAGAGAAGGTGACATCTCCGTGGGGCAGAAACCAAGCCAC TATGGTGGATGAACCTGCTGTATTTGTAAATAACCTAAAATCCTAGACCTTTTGCTCTTAGCTTTCAAGC TTATGATACAAAATGGGGAATATAAGAATTTTTTCTGTATACTGTATTTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5054239 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5054239 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17204 same embryo
	EMAGE:17206 same embryo
	EurExpress:euxassay_001611 same experiment
	MGI:4827037 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source