EMAGE mouse gene expression spatial database

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EMAGE:18070

C230030N03Rik RIKEN cDNA C230030N03 gene ( MGI:3045297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011915. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011915_01	euxassay_011915_02	euxassay_011915_03	euxassay_011915_04

euxassay_011915_05	euxassay_011915_06	euxassay_011915_07	euxassay_011915_08	euxassay_011915_09

euxassay_011915_10	euxassay_011915_11	euxassay_011915_12	euxassay_011915_13	euxassay_011915_14

euxassay_011915_15	euxassay_011915_16	euxassay_011915_17	euxassay_011915_18	euxassay_011915_19

euxassay_011915_20	euxassay_011915_21	euxassay_011915_22	euxassay_011915_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18070_wholemount_strong.wlz

18070_wholemount_moderate.wlz

18070_wholemount_weak.wlz

18070_wholemount_possible.wlz

18070_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18070_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 09 10 11 12

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 09 10 11 12

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

pons ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

spinal cord ventricular layer

strong

regional

strong expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36082
Entity Detected:	C230030N03Rik, RIKEN cDNA C230030N03 gene ( MGI:3045297)
Sequence:	sense strand is shown >T36082 CCAGCTGTCTCGTTTACTTCCTACTTCTCAAAGCAGGGCCCCTCCTTTGCAGGGTGAGTGCAAGCTGAGT GATTGTAGCTTTAAGAGGGCATCTCCAGACACACCCTGTGTAGGCCTTCCACTGGAAAACCAGGTAGAAC AGGCTTCTAGGTCATGCCCAGCCCCTTCCCACCTTACTTCAGAATTGCTGAAGCTCAACAGCCAAGAGGA AGTGCCCAGGCCGGCTGAATGCAAGCCAGGGCACAGCCCAGAAAGCAGACTTGAGGAGCCCAAAGGTGTC CACCCTGATGATGTTATCAGTAAACAACAGTGCCTGCTAAACATCTCCGGAGAGGCAGGAAGCCAGACGG AGAACTACCCATCAATGAGTGCTCTCCAGGAGGAGAGAAAGCCGCCTGATAGGGCCTCCCTCTTCCCACA ACACTGCTGCCATGGTGAGGTACCTGTGTGCAACAGAATGCTTGCAGGTTCCTGGGAAGAAGAGAGCGAT AGCGCCTGTGCCCCAAGGACAGCCCTGAAATCAAGTGTCATTGGCTCTTTAGAAGCAGCAGAAGAGGGAG TGGTTCTAGACCTCCAACGCAATGGGAAGGCCTTGCAAAGTCTTTTGGACTTCCCAACATTGGAAGTATC TCACCGCAACCCTGGGAGTGAGTGTGCACAGGGACCTGGGGATGACCCGGAGCCTTCCCCTTCTGGCCAG CGGCAGGCTCCAGGAGCCCACGGGGCCTCCAGGCACAGCTGCAAATTCATTA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67627. Forward Primer - name:067627_F_cDNA_C230030N03Rik, sequence:CCAGCTGTCTCGTTTACTTCCT; Reverse Primer - name:067627_N_SP6_cDNA_C230030N03Rik, sequence:TAATGAATTTGCAGCTGTGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18068 same embryo
	EMAGE:18067 same embryo
	EMAGE:18071 same embryo
	EMAGE:18069 same embryo
	EMAGE:18072 same embryo
	EurExpress:euxassay_011915 same experiment
	MGI:4823547 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source