EMAGE mouse gene expression spatial database

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EMAGE:18559

Slc2a12 solute carrier family 2 (facilitated glucose transporter), member 12 ( MGI:3052471)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014526. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014526_01	euxassay_014526_02	euxassay_014526_03	euxassay_014526_04

euxassay_014526_05	euxassay_014526_06	euxassay_014526_07	euxassay_014526_08	euxassay_014526_09

euxassay_014526_10	euxassay_014526_11	euxassay_014526_12	euxassay_014526_13	euxassay_014526_14

euxassay_014526_15	euxassay_014526_16	euxassay_014526_17	euxassay_014526_18	euxassay_014526_19

euxassay_014526_20	euxassay_014526_21	euxassay_014526_22	euxassay_014526_23	euxassay_014526_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18559_wholemount_strong.wlz

18559_wholemount_moderate.wlz

18559_wholemount_weak.wlz

18559_wholemount_possible.wlz

18559_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18559_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 06 07 15 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06

trigeminal v nerve

strong

regional

strong expression: see section 09 16

spinal cord

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 weak expression: see section 11 12

cervical ganglion

moderate

regional

moderate expression: see section 07 weak expression: see section 14

thoracic ganglion

weak

regional

weak expression: see section 10 11

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

neural retina

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38010
Entity Detected:	Slc2a12, solute carrier family 2 (facilitated glucose transporter), member 12 ( MGI:3052471)
Sequence:	sense strand is shown >T38010 GCATTCAAGTCCACCAGTACAAAGATTATGCAGCTTATACATCACACATGAGACACTTGTTAATCAAAGT ACTCTTTTGTAAGTTTTATGTCAATTTTTAAAACTCTAAGATAAACCATTTTCCATTTCTTTTTAAAAAT TGAGTAGAAAGATTAATTTAAAATTCCTGGGACCATATTTATTTAGAAATAGCTGTTAGTAAATATTAGA CAAGGAGTCCGGACATAATATTTATCCAGATCTCTAAGCAGTTAGCTTTAAATTATACAGTAAAAATCTC TAAGAGTTGTCTCCTGGGAAGGCTTTCGTGGTAACCTGTCACAGCCCACCTTCCTGCACTTCTTCCCTGC CCTCAGGAATCACTCTACCTCAGGCTACAGACAGAGGGATGGCCCTGACCTCAGGCCAGGTACCTTCCTT TCTGTGATGAGCAGTGATGGAGAGCCACGCCAGCAAGGTGGCTTTTACCCACGGGATGCGCTTCTCTGGC CTGCTTTTTCCATTTGAGTTTTAAGATTTGACAAAGCATTTCACCGAGAGAATTTGCATGTGATAGAAAA ATAAATCTGTGATTTAAAAAAAAGAGACCAGACCCCCTCCCTAATTTGGGTTTTTCTTCCTTTCACCACC AAATGGTCCCAGTCTGACGAGGTGTGTGCCCGCCACATGCCCTGGTAGCCAAGAGGATGAGGATGGGCAG CAGCAGGAGCCAGCGTAGAGCCTGGGCCTCAGGCGCACAGCAGGAGGCCTGCTTCTCAAGTGCAGCCTCT GAGGAAGGGAGATTTAAACCCTGATCCCATCACAGTGTGTTTCCAACTCGGGAAGAACTGTGCTGGTTCC TTTCTTGAGGGAAGAGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94164. Forward Primer - name:094164_F_cDNA_Slc2a12, sequence:GCATTCAAGTCCACCAGTACAA; Reverse Primer - name:094164_N_SP6_cDNA_Slc2a12, sequence:CCCTCTTCCCTCAAGAAAGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18556 same embryo
	EMAGE:18557 same embryo
	EMAGE:18555 same embryo
	EMAGE:18558 same embryo
	EMAGE:18554 same embryo
	EurExpress:euxassay_014526 same experiment
	MGI:4828182 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source