EMAGE mouse gene expression spatial database

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EMAGE:18627

Oxct2a 3-oxoacid CoA transferase 2A ( MGI:1891061)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016508. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016508_01	euxassay_016508_02	euxassay_016508_03	euxassay_016508_04

euxassay_016508_05	euxassay_016508_06	euxassay_016508_07	euxassay_016508_08	euxassay_016508_09

euxassay_016508_10	euxassay_016508_11	euxassay_016508_12	euxassay_016508_13	euxassay_016508_14

euxassay_016508_15	euxassay_016508_16	euxassay_016508_17	euxassay_016508_18	euxassay_016508_19

euxassay_016508_20	euxassay_016508_21	euxassay_016508_22	euxassay_016508_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18627_wholemount_strong.wlz

18627_wholemount_moderate.wlz

18627_wholemount_weak.wlz

18627_wholemount_possible.wlz

18627_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18627_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

strong

regional

strong expression: see section 04 moderate expression: see section 03 20 weak expression: see section 05 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38717
Entity Detected:	Oxct2a, 3-oxoacid CoA transferase 2A ( MGI:1891061)
Sequence:	sense strand is shown >T38717 AGCGGGAGTGTCCATTTCTACACGGACCCGGTGAAGGCGGTGGAGGGCGTCAAGGACGGGTCTACCGTCA TGCTCGGGGGCTTCGGGCTCTGCGGCATCCCCGAGAACCTGATCGGTGCGCTGAAGACCAAGGGCGTGAA GGACCTGAAGATCGTCAGCAGCAACGTGGGCGTGGACGACTTCGGCCTGGGCATCTTGCTGGCCTCCAAG CAGGTCAGGCGCGTGGTGTGCTCCTACCTGGGGGAGAACGCGCTCTGCGAGAAGCTCTACCTGGCGGGCG AGCTGGAACTGGAGATGACGCCGCAGGGAACTTTGGCCGAGCGCATCCGCGCGGGTGGCACTGGCGTGCC CGCCTTCTACACGCCCACAGGCTATGGAACGCTGGTGCAGGAAGGGGGCTCTCCAATCCGGTACGCGCCC GATGGCCACCTGATTACTCTAAGTGAGCCGCGAGAAGTACGCGAGTTCCAGGGCCGCTTCTACCTGCTAG AGCACGCTATCCGCGCTGACTTCGCACTGATCAAGGGCTGGAAGGCCGACCGCTCCGGCAACGTGATCTT CAGGGGCAGCGCGCGCAACTTCAATGTGCCCATGTGCAAGGCCGCGGACATCTCGGTGGTGGAAGTGGAG GAGATCGTGGACGTGGGTACTTTCGCCCCGGAAGACATCCACGTCCCCAACATCTATGTGGACCGCGTGA TCAAGGGGCCGAAGTTCGAGAAGCGCATCGAGCGCCTAACCACACGTGACAGCAAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104212. Forward Primer - name:104212_F_cDNA_Oxct2a, sequence:AGCGGGAGTGTCCATTTCTAC; Reverse Primer - name:104212_N_SP6_cDNA_Oxct2a, sequence:GCTTGCTGTCACGTGTGGTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18631 same embryo
	EMAGE:18630 same embryo
	EMAGE:18628 same embryo
	EMAGE:18629 same embryo
	EurExpress:euxassay_016508 same experiment
	MGI:4827010 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source