EMAGE mouse gene expression spatial database

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EMAGE:18771

E230008N13Rik RIKEN cDNA E230008N13 gene ( MGI:2685871)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014131. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014131_01	euxassay_014131_02	euxassay_014131_03	euxassay_014131_04

euxassay_014131_05	euxassay_014131_06	euxassay_014131_07	euxassay_014131_08	euxassay_014131_09

euxassay_014131_10	euxassay_014131_11	euxassay_014131_12	euxassay_014131_13	euxassay_014131_14

euxassay_014131_15	euxassay_014131_16	euxassay_014131_17	euxassay_014131_18	euxassay_014131_19

euxassay_014131_20	euxassay_014131_21	euxassay_014131_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18771_wholemount_strong.wlz

18771_wholemount_moderate.wlz

18771_wholemount_weak.wlz

18771_wholemount_possible.wlz

18771_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18771_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 02

diencephalon roof plate

moderate

regional

moderate expression: see section 10 11

choroid invagination

moderate

regional

moderate expression: see section 05 06 07 08 09 12 13 14 15 16 17

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39840
Entity Detected:	E230008N13Rik, RIKEN cDNA E230008N13 gene ( MGI:2685871)
Sequence:	sense strand is shown >T39840 CCAGGAACAAGAGGAATTCAAGAAGAGGAAGTACCTGAGCGCACTGGCGAGCTTTCAGGAGGAGTTGGAG CAGATTGGGGTGGAGATGGAACCTCTCATCCTGGAGAAAGGGGATCTTCTTTTGAGAAAGATCTCGAAGT CCGATGAGGACGTTGACATGCTCTTCAAGAAAGTCGAAGGGGAGCCGGACCTCGAAAGCCACACTATTGA AGTAGGGGCTCCCTGGTGACAACACCTGCTCCCCACCTCTCCGGGAACTCCTGGCCTGATCCTGTTCCCC TTTGCAGACCCTGTTGGAACTGTGGGAAAAAGTGGCTGAAAGATTCATGCAGCAGAAGCAGGAGATTAAG GAGCTGGACGAGGAGCTGTTGGCCCTGGAGGTCTCTCGAGCCGACAAGCTAAAGGAAGTGCTGAAGAGAT ATGTCGGCATCATAGAGAAAACCTCCTATATCCTGCAGCCCGATGTGTATAGGCTGATAGATAAAGAAGC TATGGCCATGAACCAGGCTCTCCTGGGCAACCGGAGGGCCATCGCCCAGCTGTTAGTCAACCTGACTGAG TCAACCCTACAGCAGGAGCTTGACAACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 264927. Forward Primer - name:264927_F_cDNA_E230008N13Rik, sequence:CCAGGAACAAGAGGAATTCAAG; Reverse Primer - name:264927_N_SP6_cDNA_E230008N13Rik, sequence:GGTTGTCAAGCTCCTGCTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18772 same embryo
	EMAGE:18775 same embryo
	EMAGE:18776 same embryo
	EMAGE:18773 same embryo
	EMAGE:18774 same embryo
	EMAGE:18777 same embryo
	EurExpress:euxassay_014131 same experiment
	MGI:4824450 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source