Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18839

Sco1 SCO cytochrome oxidase deficient homolog 1 (yeast) ( MGI:106362)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839
"Pseudo-wholemount" of euxassay_014186. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014186_01 euxassay_014186_02 euxassay_014186_03 euxassay_014186_04
EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839
euxassay_014186_05 euxassay_014186_06 euxassay_014186_07 euxassay_014186_08 euxassay_014186_09
EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839
euxassay_014186_10 euxassay_014186_11 euxassay_014186_12 euxassay_014186_13 euxassay_014186_14
EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839
euxassay_014186_15 euxassay_014186_16 euxassay_014186_17 euxassay_014186_18 euxassay_014186_19
EMAGE:18839 EMAGE:18839 EMAGE:18839 EMAGE:18839
euxassay_014186_20 euxassay_014186_21 euxassay_014186_22 euxassay_014186_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18839Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18839_wholemount_strong.wlz
18839_wholemount_moderate.wlz
18839_wholemount_weak.wlz
18839_wholemount_possible.wlz
18839_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18839_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40565
Entity Detected:Sco1, SCO cytochrome oxidase deficient homolog 1 (yeast) ( MGI:106362)
Sequence:sense strand is shown

>T40565
CTTTAGCTCTCACCTTTGCCATTGGAGGCTCTTTATTGGCTGGAATGAAGTACTTCAAGAAAGAAAAGAT
AGAAAAGCTGGAGAAACAACGGCATCGCAGCATTGGGAAGCCTTTACTAGGGGGGCCATTTTCCCTTACA
ACTCACAATGGAGAGCCCAAAACCGACAAGGACTACCTGGGTCAATGGGTATTGATTTATTTTGGCTTCA
CTCATTGCCCTGATATCTGTCCAGAAGAACTAGAAAAAATGATTGAAGTCGTGGAGGAGATAGACAGTAT
TCCATCCCTGCCAAATTTAACTCCACTTTTCATCACCATTGACCCAGAAAGGGACACCAAAGAAGCCATT
GCGACTTATGTGAAAGAATTTTCTCCCAAATTGGTTGGTTTGACTGGCACAAAAGAAGAGATTGATGGAG
TGGCCAGAGCATACAGGGTGTATTACAGCCCTGGCCCGAAGGATGAGGATGAAGACTACATAGTGGATCA
TACAATAATAATGTACTTGATTGGACCAGATGGAGAATTTCTTGATTATTTTGGACAAAACAAGAAGAAG
GCAGAAATAGCCGGCTCAATTGCTGCACACATGAGGTCACACATGAAGAAGAGGTAGCCAGAGCCGTGTC
ACCAGATGCAGGATTGGCTTGCTGAAGAGGAAGCTTTGTCTCCCAGAGGAACCTCTATATACTGTAACAT
ACAACATGGACTTCATACCCTGAGAACATCATTCTACATGAGATGTTACCCTGGGGCTCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 161527. Forward Primer - name:161527_F_cDNA_mCG13452.1, sequence:CTTTAGCTCTCACCTTTGCCAT; Reverse Primer - name:161527_N_SP6_cDNA_mCG13452.1, sequence:CTGAGCCCCAGGGTAACATCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18842 same embryo
 EMAGE:18840 same embryo
 EMAGE:18841 same embryo
 EMAGE:18838 same embryo
 EMAGE:18843 same embryo
 EurExpress:euxassay_014186 same experiment
 MGI:4827907 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS