EMAGE mouse gene expression spatial database

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EMAGE:18968

Isg20l2 interferon stimulated exonuclease gene 20-like 2 ( MGI:2140076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015937. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015937_01	euxassay_015937_02	euxassay_015937_03	euxassay_015937_04

euxassay_015937_05	euxassay_015937_06	euxassay_015937_07	euxassay_015937_08	euxassay_015937_09

euxassay_015937_10	euxassay_015937_11	euxassay_015937_12	euxassay_015937_13	euxassay_015937_14

euxassay_015937_15	euxassay_015937_16	euxassay_015937_17	euxassay_015937_18	euxassay_015937_19

euxassay_015937_20	euxassay_015937_21	euxassay_015937_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18968_wholemount_strong.wlz

18968_wholemount_moderate.wlz

18968_wholemount_weak.wlz

18968_wholemount_possible.wlz

18968_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18968_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 12 13 14

submandibular gland primordium

weak

regional

weak expression: see section 09 10 11 17 18 19

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 10 11 12 13

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 weak expression: see section 13

dorsal root ganglion

moderate

regional

moderate expression: see section 16 weak expression: see section 09 10 11

lower jaw incisor

weak

regional

weak expression: see section 13 14 17

lower jaw molar

weak

regional

weak expression: see section 08 20

upper jaw incisor

weak

regional

weak expression: see section 13 14 17

upper jaw molar

weak

regional

weak expression: see section 08 19 20

metanephros

weak

regional

weak expression: see section 04 05 06 07 08 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63280
Entity Detected:	Isg20l2, interferon stimulated exonuclease gene 20-like 2 ( MGI:2140076)
Sequence:	sense strand is shown >T63280 CGCTACACCCTTTAAGACTGCTCGGAGTCAGATCTTGAAGATACTCTCAGGGAAGGTAGTGATAGGGCAT GCCATTCACAATGACTACAAAGCCCTACAGTACTTTCATCCCAAGTCCCTCACCAGAGACACTTCCCGGA TACCACTCCTCAACCGGAAGGCTGACTGCCCAGAAAATGTCACTTTGTCACTGAAGCATCTCACCAAGAA GCTCCTGAGTCGGGACATCCAGGTTGGAAATACTGGACATTCCTCGGTGGAAGATGCCCAGGCCACAATG GAGCTGTACAAGTTGGTTGAAGTTGAATGGGAACAGCACCTGGCCCAGAATCCTCCAGAAAATTAGCAAT CCCAGGGAGACACATGCAGTAGACCAACCCACAGTTCTTCCAGCTTCTCACCTGCAGAAGCTAGAATCAC TGGGGAGAGGGGCTTGGCCAGAGGCTTAATACCCATTGAAATTTCATTTCAGCTATTTTGGTCACTGTGT GGTTAAGTGTCTCATGGAAGAGGAGAGCTTTCATGTTAAACCCACCTCTAGGTCAGGCAGGTGGCTCAGA CCTGTAATCCCAGATACTTGGGAGGCTGAAACAGGTTATAGATGTAAAGTCTGCCTGGACTACAGAATTA ATTCTAGGTCAGTGAGACCCTGTCCTAACATAAAAAGAGGGCCAGGGGTAAAGCCCAGCGCTAGAACACT TACCTAGCATGCATAAACCACTAGGTACAGTCTCCAGCAGTGGGGTGGGGGAGGGTAGAAGGAATGGAAT ACAAAGTCATGAACCATAACTGTTTACTTCTTTAGAGTGCTAACCACATGTCACAGGGTGCTTGTGCCAG TCAGCCTTGACTTTGAGGAAGCAAGGCACACCAGGGAAAACAGTGTCCCACACTACCTTCTCTATGGGAC ACATGTCATAGATATGTCTCCTTTTGGTGGGGAGTAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70277. Forward Primer - name:070277_F_cDNA_C80587, sequence:CGCTACACCCTTTAAGACTGCT; Reverse Primer - name:070277_N_SP6_cDNA_C80587, sequence:CTACTCCCCACCAAAAGGAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18966 same embryo
	EMAGE:18965 same embryo
	EMAGE:18964 same embryo
	EMAGE:18967 same embryo
	EMAGE:18963 same embryo
	EurExpress:euxassay_015937 same experiment
	MGI:4825629 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source