EMAGE mouse gene expression spatial database

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EMAGE:18993

Inpp5a inositol polyphosphate-5-phosphatase A ( MGI:2686961)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015905. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015905_01	euxassay_015905_02	euxassay_015905_03	euxassay_015905_04

euxassay_015905_05	euxassay_015905_06	euxassay_015905_07	euxassay_015905_08	euxassay_015905_09

euxassay_015905_10	euxassay_015905_11	euxassay_015905_12	euxassay_015905_13	euxassay_015905_14

euxassay_015905_15	euxassay_015905_16	euxassay_015905_17	euxassay_015905_18	euxassay_015905_19

euxassay_015905_20	euxassay_015905_21	euxassay_015905_22	euxassay_015905_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18993_wholemount_strong.wlz

18993_wholemount_moderate.wlz

18993_wholemount_weak.wlz

18993_wholemount_possible.wlz

18993_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18993_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mandible

weak

regional

weak expression: see section 06 07 08 09 10 16 17 18 19 20 21 22

maxilla

weak

regional

weak expression: see section 07 08 09 10 11 18 19 20 21

orbito-sphenoid

weak

regional

weak expression: see section 03 04 05 06 07 21 22 23

clavicle

weak

regional

weak expression: see section 09 10 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63248
Entity Detected:	Inpp5a, inositol polyphosphate-5-phosphatase A ( MGI:2686961)
Sequence:	sense strand is shown >T63248 ATGAACTGGACATCTCATTCCCCCCCAGCTACCCGTACAGTGAGGACTCCAGCCAGGGAGAACAGTACAT GAACACGAGATGCCCTGCTTGGTGTGATCGCATCCTCATGTCCCTGTCTGCCAAGGAGCTGGTTCTTAAG TCAGAGAGCGAGGAGAAGGTTGCCACCTACGACCACATCGGGCCTAATGTCTGCATGGGAGACCACAAGC CGGTGTTCCTGGCCTTCCGAATCGCACCTGGGGCAGGGAAGAGGTGCCAGCGCCGCGAGAGGATCCTCGA GAGGCCTCCCTGTAGCAGTGTATCCAACTCATCCTCCTAACAGCTGCATCGGAACCGCCCAAGCCTCACC TGCTAGACGGCCGGCCCCACACTTCGCTTCAGCCTCCGGACCTCCGGAAAGCCTCACATACCTCACTGTC GTGTCTGTCACGTGACATTGTAGACATATTGGGTGTAATCAAGCCACAGTCCTATTGCCAAAACTCTAAC AGACAGCAGAGCGCCTGTATTTTAGACTTCACAGTTTTCCATTTTTAACAATTGTCTGTGGAGGAAGCTT CTCCATTTAGAGCCCCAGGTGTCCAGCGTCCCTCTCTGCCAGGAGGAGTGTGCCGGGCACTAGCCCAGGC AGCCAGGCTGCCACGTGTGCCCAAGCCCGAGGCCCGAGGTCTGACTCTACTGCCATGTTACGTGGCGTGA GAATCACACTGCAGCTGCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95809. Forward Primer - name:095809_F_cDNA_Inpp5a, sequence:ATGAACTGGACATCTCATTCCC; Reverse Primer - name:095809_N_SP6_cDNA_Inpp5a, sequence:AAGCAGCTGCAGTGTGATTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18998 same embryo
	EMAGE:18996 same embryo
	EMAGE:18997 same embryo
	EMAGE:18995 same embryo
	EMAGE:18994 same embryo
	EurExpress:euxassay_015905 same experiment
	MGI:4825595 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source