EMAGE mouse gene expression spatial database

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EMAGE:19791

Hps4 Hermansky-Pudlak syndrome 4 homolog (human) ( MGI:2177742)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006298. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006298_01	euxassay_006298_02	euxassay_006298_03	euxassay_006298_04

euxassay_006298_05	euxassay_006298_06	euxassay_006298_07	euxassay_006298_08	euxassay_006298_09

euxassay_006298_10	euxassay_006298_11	euxassay_006298_12	euxassay_006298_13	euxassay_006298_14

euxassay_006298_15	euxassay_006298_16	euxassay_006298_17	euxassay_006298_18	euxassay_006298_19

euxassay_006298_20	euxassay_006298_21	euxassay_006298_22	euxassay_006298_23	euxassay_006298_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19791_wholemount_strong.wlz

19791_wholemount_moderate.wlz

19791_wholemount_weak.wlz

19791_wholemount_possible.wlz

19791_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19791_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midgut

moderate

regional

moderate expression: see section 11 14 15

midgut loop

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 weak expression: see section 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8444
Entity Detected:	Hps4, Hermansky-Pudlak syndrome 4 homolog (human) ( MGI:2177742)
Sequence:	sense strand is shown >T8444 TCCACCTCCAGTCCTCCTGGACTTGTCAGCAGTGCAGAGATACTTCTGGAAAGGAACCTGCCCCGAAGGG AAGTTGCCTTTTCATATTGATTCAAGAGTGAACAAGAGGCAGAAACCACCTGGATGGTCCACACCTGGCA TCACTGAGCTGGGGACACCAGTTGGGGGACAGAGAACTGCTTCTAGGAAGAACACCAGATGAAGAGGATG AAGCCATCTGGAGAGTAAATGGCCACCACCACTCCCCCAGAGACAAAGTCAGCAGCGTGGTGGAACTACT TCTTCCTGTATGACGGTTCCAAGGTAAAGGGAGAAGGAGACCCAACCAGAGCTGGCATCTGCTACTTCTA TCCGCCCCAGACCCTGTTAGACCAGCAGGAACTGCTCTGTGGCCAGCTTGCTGGAGTAGTGCGCTGCCTG TGGGACCTTTCTGGCACCCCGCCCATGCTCATCCGTATGCGGAACCTCAAGTTTGCCATCAGGGCAGATG GGGACTACCTGTGGGCTCTGGGGTGTGGTGTGGAGATCTCTGATGCCAGCTGCAGGCAGTTTCTGGATCA GCTCATTGGATTTTTTCATTTCTACATGGGCCCTGTCTCCCTGGCCTACAAGAGCCATCCTCAGGAGGAG CTGAGCTTGCAGTGGGATACCTCCATCACACAAGTCCTCAGGAGCACCAGCGAATCGCACAGGATCTTCA ATGCCCTGTGGAACCTGGACCGCACCAAAGTGGAGCCCCTGCTGCTGCTGAAGGCAGCTCTCATCTTACA GACCTGCCAACGCTCACCTCATGTGCTAGCTGGCTGCATCCTCTATAAAGGACTGATTGTGAATTCCCAG CTCCTGCCTTCCCTCACTGCTAAGGTCCTGCTTCATCAGACTGTACCTGCAGACCAGAGGCTGCCTGGAG CAGGAGCTGCCCCACAGGAAACAGGAGCAGCGTTGCCCCCGGATGTGCAGATCACCTCTGTTTTTCTGAG TGAGGAGGAAGCTGCCAGCCTCCACGAGTTCCCAGTAGAGCATGAAACTCGACTCCAGGGAAGTTCAGTT CAATACCCTCCCTGGGATCAAAGCAGCCCCACCCAGGCAGAAGATGCCTGGGCATCAGCTGCCATCCCGG AGCCCACGCCGCATGATGGAGCATGTCCGAGTGGCAGCGGGGCAGATGAACGCTTGCCTAGGCTTGAGCA GGAGTGTGCTGGGCCCACGGGACTGTGCACCACTGCCTGTGGCCAGGGCTCCGGCCTCAGCAGCAGGCTG CAGAAGGAGCTGTGTCTCTCCCGGGAGGAACTGGACTCGTCTGAAATGCACGTTTCTGAGGCTCAGGAAG CCTTCCCACCTTTGCCTGCCCTGGGTGATCTGGAGACTCTCCACAGCAGCCACAGTGCCCCCACTCTACC TGAAGACACAGCCATTTGCAGCTGCCTGCACCCTTGCCCTCTTGAGAGGCTCCCTGAGAGTGGAAGGCTA GGACAGCTCGCAGACCTGCCCCTCACCAATGGCCAAACCCAGGTTCCTGGCACAGACCCTCTCCCCAGCA GCATGCCTGTGGCACTTCCTCCCCAGCATCCTGTGGGTGTAGAGCCCAGTGTGGAGCCATACGGGAATGG AGCCCAGGAGAGCCACTCAGCCCTGCCACGCAGTTCCCGCTCACCTGACTCTCCGGGCCCCAGCCCCTCT GCAGACAGAACTGGCTTCAAGCCATCCCCATCTGGACGCCACGCAGGGCTCGTGCCCATGAACCTCTACA CCCACAGTGTGAACGGACTGGTGCTGTCCCTGCTGGCCGAAGAGACTCTTCTCAGCGACACTGCAGCCAT CGAGGAGGTGTACCACAGCAGCCTGGCGTCGCTGAATGGGCTGGAAGTCCACCTGAAGGAGACGCTGCCC AGAGACGAGGCTAGCCTCACCAGCAGCACATACAACTTCCTTCATTATGACCGCATCCAGAGCGTGCTCT CAGCCAACCTGCCCCTAGTGACTGCTCCCCAGGACCGCCGGTTTCTGCAGGCCGTCAACCTCATGCACTC CGACTTTGCCCTGCTGCCTATGCTGTACGAGATGACCATCAGGAACGCCTCCACAGCCGTGTACGCCTGC AGCAGCCCAGCCCAGGAGACCTACTTCCAGCAGCTGGCACCCACTGCCCGCAGCTCTGGCTTCCCAAACC CTCAGGATTGCGCCTTCAGCCTCGCAGGCAAAGCCAAGCAGAAGCTGCTGAAGCACGGGGTAAACCTGCT GTGAGCTGGGTGCAGCTTGCATATCACGAGGGCCATGTCCCCAATGCTAACGGTTGAGCGGGCCCTTGCT CCTCAGTGGCAGCTGCAAGAACTGAGTGGGCTCAGGTCCTGTCCTGTGGAAGCCTTGAGGGCGGTGCAGA GCAGGCTGCAGGCTCAGGAATGCAGCGGCAGGATTCACTGCTGACCGTTTACTTGAGATACCCATGGTGC TTTGTAACCGTATGGTGCCCAGTTCCAGGTTTATGAAGCAGATTCCCAAAGGCTCAAAAATAAAATGACC AAAGTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5701206 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5701206 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19790 same embryo
	EurExpress:euxassay_006298 same experiment
	MGI:4825447 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source