EMAGE mouse gene expression spatial database

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EMAGE:20031

Slc7a2 solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 ( MGI:99828)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012150. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012150_01	euxassay_012150_02	euxassay_012150_03	euxassay_012150_04

euxassay_012150_05	euxassay_012150_06	euxassay_012150_07	euxassay_012150_08	euxassay_012150_09

euxassay_012150_10	euxassay_012150_11	euxassay_012150_12	euxassay_012150_13	euxassay_012150_14

euxassay_012150_15	euxassay_012150_16	euxassay_012150_17	euxassay_012150_18	euxassay_012150_19

euxassay_012150_20	euxassay_012150_21	euxassay_012150_22	euxassay_012150_23	euxassay_012150_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20031_wholemount_strong.wlz

20031_wholemount_moderate.wlz

20031_wholemount_weak.wlz

20031_wholemount_possible.wlz

20031_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20031_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lens

strong

regional

strong expression: see section 01

viscerocranium

weak

regional

Expression in the turbinate bone.

mandible

strong

regional

strong expression: see section 03 04 05 21 22 23 moderate expression: see section 06 07 08 09 14 15 16 17 18 19 20 weak expression: see section 10 11

maxilla

strong

regional

strong expression: see section 03 04 05 21 22 23 moderate expression: see section 06 07 08 09 14 15 16 17 18 19 20 weak expression: see section 10 11

ovary

weak

regional

weak expression: see section 05 06 19 20

trachea

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37044
Entity Detected:	Slc7a2, solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 ( MGI:99828)
Sequence:	sense strand is shown >T37044 GGGCTTGAGTATTCTAACCACGTATGGCGTCCAGGCCATTGCCAGACTGGAAGCCTGGAGCCTGGCTCTT CTCGCCCTGTTCCTTGTCCTCTGCGCTGCCGTCATTCTGACCATTTGGAGGCAGCCACAGAATCAGCAAA AAGTAGCCTTCATGGTCCCGTTCTTACCGTTTCTGCCGGCCTTCAGCATCCTGGTCAACATTTACTTGAT GGTCCAGTTAAGTGCGGACACTTGGATCAGATTCAGCATCTGGATGGCGCTTGGCTTTCTGATCTATTTC GCCTATGGCATTAGACACAGCTTGGAGGGTAACCCCAGGGACGAAGAAGACGATGAGGATGCCTTTTCAG AAAACATCAATGTAGCAACAGAAGAAAAGTCCGTCATGCAAGCAAATGACCATCACCAAAGAAACCTCAG CTTACCTTTCATACTTCATGAAAAGACAAGTGAATGTTGATGCTGGCCCTCGGTCTTACCACGCATACCT TAACAATGAGTACACTGTGGCCGGATGCCACCATCGTGCTGGGCTGTCGTGGGTCTGCTGTGGACATGGC TTGCCTAACTTGTACTTCCTCCTCCAGACAGCTTCTCTTCAGATGGTGGATTCTGTGTCTGAGGAGACTG CCTGAGAGCACTCCTCAGCTATATGTATCCCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89486. Forward Primer - name:089486_F_cDNA_Slc7a2, sequence:GGGCTTGAGTATTCTAACCACG; Reverse Primer - name:089486_N_SP6_cDNA_Slc7a2, sequence:TGGGGATACATATAGCTGAGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20028 same embryo
	EMAGE:20030 same embryo
	EMAGE:20029 same embryo
	EMAGE:20027 same embryo
	EMAGE:20032 same embryo
	EurExpress:euxassay_012150 same experiment
	MGI:4828274 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source