EMAGE mouse gene expression spatial database

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EMAGE:20201

Ppp1r9a protein phosphatase 1, regulatory (inhibitor) subunit 9A ( MGI:2442401)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012258. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012258_01	euxassay_012258_02	euxassay_012258_03	euxassay_012258_04

euxassay_012258_05	euxassay_012258_06	euxassay_012258_07	euxassay_012258_08	euxassay_012258_09

euxassay_012258_10	euxassay_012258_11	euxassay_012258_12	euxassay_012258_13	euxassay_012258_14

euxassay_012258_15	euxassay_012258_16	euxassay_012258_17	euxassay_012258_18	euxassay_012258_19

euxassay_012258_20	euxassay_012258_21	euxassay_012258_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20201_wholemount_strong.wlz

20201_wholemount_moderate.wlz

20201_wholemount_weak.wlz

20201_wholemount_possible.wlz

20201_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20201_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

single cell

strong expression: see section 01 02 03 04 05 21 22

upper leg muscle

strong

single cell

strong expression: see section 08 moderate expression: see section 07 09 15 16 weak expression: see section 17

hypothalamus mantle layer

moderate

single cell

moderate expression: see section 11 12 weak expression: see section 15

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 09 16 17 18

pons mantle layer

moderate

single cell

moderate expression: see section 10 16 17 18

pons ventricular layer

moderate

single cell

moderate expression: see section 10 16 weak expression: see section 13 15

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 09 10 11 12 13 15 16 17 18 19

midbrain ventricular layer

moderate

single cell

moderate expression: see section 10 11 12 16 weak expression: see section 17

tongue muscle

weak

regional

weak expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30392
Entity Detected:	Ppp1r9a, protein phosphatase 1, regulatory (inhibitor) subunit 9A ( MGI:2442401)
Sequence:	sense strand is shown >T30392 CCATGCTTGGATCAGCGTACATCTGGCAGTTACAAATCAAACTAATGTTCCTTACATCATTAGTTTATAG TTTAGTCATTGTAATCTTTAAGTTAAATTATTGCAATCTTAAACCAAAATGAGTTCTTTTAACGTTGCTA CTTAAAACTAGTAAAATCGTAAAGGGCTCTTGATTGCACTGTTCAGCAAAGGATAAGAGATGGACTGGTT GGTCCGGTGTGGAAACAAAGTGAAGGACCTCAGATCTTGTGAGCGTTTTGACTTCTTGTTAGCTCCTTTA TTGGCTGTAGTGCATGATAATAATTGAGTTTCCAACGTCTTGCACAATGCTTTCTTTAATGCTGTGGTTT TCAGGGTTTATACAACTTAAAGAGAATTGTTGCTTCATGGACTTTAAGGATTTCGGACAGTTTTTAAGCT ATCAACAAATGAACAGAAGGGGTTGTCTGCATATTGTGAACTGTACTCTAGTGAAGACTAGTGGGTCCCA CCAGAAACCAAAAGTGTTTGGGGTACAATATATGTTCGGGTGTCAGGGTACATGGTGAGGCCCTGCCATA GGTAGAGAGCATTGTAGGAGTAGATTCTGGGTCAGGGTTCCACTAAAGAACTGTGCACTGGATGTCTGTG ATTAAGATCATCTCCTTTAAGGCAACTTATCCTTATGTGATCACGTCTTTCTCCTCCCCAGCATCAGGAG GGTACTGTTAGAGCAGAAACCCTGCTCTATCTACTTTAGCACTCGATCGAAGCCTGCCATTACTATCGTG ACAGCAAAGTCCCTCTGAAACCATCTGCCATCCTTCTGCTGGGGTTGGGATGGACTTCCTGCTCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6331919), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58625. Forward Primer - name:058625_F_IRAV102_h12_Ppp1r9a, sequence:CCATGCTTGGATCAGCGT; Reverse Primer - name:058625_R_SP6_IRAV102_h12_Ppp1r9a, sequence:GGGGAGCAGGAAGTCCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20198 same embryo
	EMAGE:20200 same embryo
	EMAGE:20199 same embryo
	EMAGE:20197 same embryo
	EurExpress:euxassay_012258 same experiment
	MGI:4827380 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source