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EMAGE:20257

Ube2j1 ubiquitin-conjugating enzyme E2, J1 ( MGI:1926245)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257
"Pseudo-wholemount" of euxassay_012405. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012405_01 euxassay_012405_02 euxassay_012405_03 euxassay_012405_04
EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257
euxassay_012405_05 euxassay_012405_06 euxassay_012405_07 euxassay_012405_08 euxassay_012405_09
EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257
euxassay_012405_10 euxassay_012405_11 euxassay_012405_12 euxassay_012405_13 euxassay_012405_14
EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257
euxassay_012405_15 euxassay_012405_16 euxassay_012405_17 euxassay_012405_18 euxassay_012405_19
EMAGE:20257 EMAGE:20257 EMAGE:20257 EMAGE:20257
euxassay_012405_20 euxassay_012405_21 euxassay_012405_22 euxassay_012405_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20257Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20257_wholemount_strong.wlz
20257_wholemount_moderate.wlz
20257_wholemount_weak.wlz
20257_wholemount_possible.wlz
20257_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20257_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 15 16 weak expression: see section 08 09
pons mantle layer
weak weak
regionalweak expression: see section 06 17
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 19 20 21
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 07 17 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 08
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 12 13 16 17 weak expression: see section 06 14 15
neural retina
weak weak
regionalweak expression: see section 02 03 23
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 12 14 15 16 17 18 19
mandible
weak weak
regionalweak expression: see section 04 06 07 19 20 21 22 23
lower jaw incisor
weak weak
regionalweak expression: see section 12 13 16 17
upper jaw incisor
weak weak
regionalweak expression: see section 12 13 16 17
orbito-sphenoid
weak weak
regionalweak expression: see section 03 04 05 21 22
clavicle
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31270
Entity Detected:Ube2j1, ubiquitin-conjugating enzyme E2, J1 ( MGI:1926245)
Sequence:sense strand is shown

>T31270
TGGAGACCCGCTACAACCTGAAGAGCCCGGCCGTTAAGCGATTGATGAAAGAAGCTGCAGAATTGAAAGA
TCCAACAGATCATTACCACGCTCAGCCTCTGGAGGATAACCTTTTTGAATGGCACTTCACAGTTAGAGGG
CCCCCAGATTCCGATTTTGATGGAGGAGTTTACCATGGACGCATAGTACTGCCACCAGAGTACCCCATGA
AACCACCAAGCATTATCCTGCTAACAGCTAATGGACGATTTGAAGTAGGCAAGAAGATCTGTTTAAGCAT
CTCAGGACATCATCCTGAAACCTGGCAGCCTTCATGGAGCATAAGGACAGCCTTGCTGGCCATCATCGGG
TTTATGCCGACTAAAGGAGAGGGGGCCATAGGTTCTCTAGATTACACACCAGAGGAGAGAAGAGCGCTTG
CCAAAAAATCGCAAGATTTCTGTTGTGAAGGCTGTGGCTCTGCCATGAAGGATGTCCTGTTGCCATTAAA
ATCTGGAAGTGGTTCAAGCCAGGCTGACCAAGAAGCCAAAGAGCTGGCCAGACAGATTAGTTTTAAGGCC
GAAGTCAATTCATCTGGAAAGACTATCGCTGAATCAGACTTAAACCAGTGTTTCTCACTAAATGACTCAC
AAGACGATTTACCTACAACATTCCAGGGTGCTACAGCAAGCACATCGTATGGCGCCCAGAATCCATCAGG
AGCACCCTTGCCACAACCTACCCAGCCAGCTCCTAAGAATACCTCCATGAGCCCTCGGCAGCGCAGAGCC
CAGCAACAGAGTCAGAGAAGGCCATCCACTTCCCCCGATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4217620), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 54958. Forward Primer - name:054958_F_IRAV49_g04_Ube2j1, sequence:TGGAGACCCGCTACAACC; Reverse Primer - name:054958_R_SP6_IRAV49_g04_Ube2j1, sequence:ACATCGGGGGAAGTGGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20256 same embryo
 EMAGE:20258 same embryo
 EurExpress:euxassay_012405 same experiment
 MGI:4829054 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS