EMAGE mouse gene expression spatial database

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EMAGE:20449

1700009P17Rik RIKEN cDNA 1700009P17 gene ( MGI:1922722)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012653. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012653_01	euxassay_012653_02	euxassay_012653_03	euxassay_012653_04

euxassay_012653_05	euxassay_012653_06	euxassay_012653_07	euxassay_012653_08	euxassay_012653_09

euxassay_012653_10	euxassay_012653_11	euxassay_012653_12	euxassay_012653_13	euxassay_012653_14

euxassay_012653_15	euxassay_012653_16	euxassay_012653_17	euxassay_012653_18	euxassay_012653_19

euxassay_012653_20	euxassay_012653_21	euxassay_012653_22	euxassay_012653_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20449_wholemount_strong.wlz

20449_wholemount_moderate.wlz

20449_wholemount_weak.wlz

20449_wholemount_possible.wlz

20449_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20449_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon floor plate

weak

regional

weak expression: see section 13

lateral ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata floor plate

weak

regional

weak expression: see section 12

metencephalon floor plate

weak

regional

weak expression: see section 12

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 moderate expression: see section 20

midbrain floor plate

weak

regional

weak expression: see section 12

spinal cord floor plate

weak

regional

weak expression: see section 11

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 weak expression: see section 13 16 17 18 19

heart ventricle

moderate

spotted

moderate expression: see section 07 08 09 weak expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31935
Entity Detected:	1700009P17Rik, RIKEN cDNA 1700009P17 gene ( MGI:1922722)
Sequence:	sense strand is shown >T31935 CCAGGCCAACGAAAGAGAAAATCGCTGCCCATGAAGGTTACACTCAGATCATCGCCAACGATCGAGGGCA TCTCTTGCCTTCAGTGCCCCGTTCCAAGGCAAGTCCTTGGGGTTCCTTCATGGGCACCTGGCAAATGCCC CTGAAGATCCCACCTGCTAAGGTGACCTTGACCGCCCGTACAACTACAGCTGCCGACAACCTCACCAAAT GGATACACAAGAATCCTGATCTACTCAACGCCTGTAATGGGCTGCGTCCTGAAATCTCAGGCAAGCCCTT CGATCCTGACAGTCAGACGAAACAGAAGAAATCTGTCACCAAGACTGTACAACAAGCACCAAATCCAACC ATAATTCCCAGCTCCCCGGTTATCCAAGGAGACAACCCAGATGAACCGCAAAGCTCGCACCCCTCTGCAG GTCACACTCCAGGTCCCCAAACTCCAGTCAACTCTCCCAACAACCCACCTCCGAGCCCTTGTAAGAGCAC CAAGTAGGTCCTAGTCTAGCTGAGGTCCAGATCTATGCCATGCTGGGGTGACATTTTAGAGACTGACCGA AATAGGTGAGACCCTGCCTGTATTCAGAAATGTGGAACAGAGAAATGGTGGGCCAGGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6774925), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61225. Forward Primer - name:061225_F_IRAW6_c11_1700009P17Rik, sequence:CCAGGCCAACGAAAGAGA; Reverse Primer - name:061225_R_SP6_IRAW6_c11_1700009P17Rik, sequence:ACTCCTGGCCCACCATTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20451 same embryo
	EMAGE:20450 same embryo
	EMAGE:20446 same embryo
	EMAGE:20448 same embryo
	EMAGE:20447 same embryo
	EurExpress:euxassay_012653 same experiment
	MGI:4822622 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source