EMAGE mouse gene expression spatial database

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EMAGE:20640

Lrrtm4 leucine rich repeat transmembrane neuronal 4 ( MGI:2389180)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013925. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013925_01	euxassay_013925_02	euxassay_013925_03	euxassay_013925_04

euxassay_013925_05	euxassay_013925_06	euxassay_013925_07	euxassay_013925_08	euxassay_013925_09

euxassay_013925_10	euxassay_013925_11	euxassay_013925_12	euxassay_013925_13	euxassay_013925_14

euxassay_013925_15	euxassay_013925_16	euxassay_013925_17	euxassay_013925_18	euxassay_013925_19

euxassay_013925_20	euxassay_013925_21	euxassay_013925_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20640_wholemount_strong.wlz

20640_wholemount_moderate.wlz

20640_wholemount_weak.wlz

20640_wholemount_possible.wlz

20640_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20640_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 10 11 13 15 16 17 weak expression: see section 14

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 18 19 20 21 22 weak expression: see section 09 10 11 13 14 15 16 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 07 09 10 11

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 15 16

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 15 16

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 08

pons mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

midbrain mantle layer

moderate

regional

moderate expression: see section 06 18 weak expression: see section 15 16 17

tegmentum

moderate

regional

moderate expression: see section 08 09 10 11 13 14 weak expression: see section 15

spinal cord mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38226
Entity Detected:	Lrrtm4, leucine rich repeat transmembrane neuronal 4 ( MGI:2389180)
Sequence:	sense strand is shown >T38226 GGCAGTAACATAATGACAGGCATGACAGAGCCTGCTTGTCATCTTTGCTGTCTTCCTCAAAAGGAAAGAC AGAAACTCTTGTTAAATTGACCCAAAACTAAGTGATGCAAATATTTATGAATAAAAGGTGTAACTTTGGC TGGGGTCTTTTTTATTTGCATAGAATCCTCAAAGAGATGTCTGAATTTCATGTTGGACTCTTTTCATTTA TATGAGAATAAGTTTGGTCCAGTTTCTGAGTATCTAATTTAAAAGATCACTTACAAAAACACATTACATG AATGGGATGGTATTTATCCACTTCCATTTTCTGATTGAACTATATTGGATATATGTTGTTTTTTCATTGG TAAAATAATACTATGTAACAGCCAACTGTAAGGTTTTGTTGCCAGGATACTTTAGCTGATAGAAGGTTGT TCTGGCAATTGACTCTTTTAGTACCTGTTATTAGATGGTATTTTCAAAAGTGAGTTTTAATGAGTGTTTA AAGATGGTGCTCTTTCTAATGTTACTTATAACAGTTAGGAAACATAATTTGTTCTTTGGTAACATATACC AAGATTTCCACAGACCTAAATACATATGTTAATGGGAGTGTTTTCAGATGCTACAGTGAATTTAAAATAA TCTCAAACTTAGTACAGTTTGGACTTTTTGAACTGTTCTTTGCTGAGTTTGTTTTCTCTTTCTTGCTCTC TTTTTTTTTTCTCATTTGTGTACTATTGAAATAAAAGACATAATCCCAAATAATAAACCCCCCAAAGAAA CCTGTATAAGTGATTGCCCTTAAAATTTAAAGAGAATAAAAACTTTCTATGAAAGTCTACCAAGGCTTGA TGTTTTTCTTGCAAGCCCACATTGACACAGTAGAGGTGAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 142975. Forward Primer - name:142975_F_cDNA_Lrrtm4, sequence:GGCAGTAACATAATGACAGGCA; Reverse Primer - name:142975_N_SP6_cDNA_Lrrtm4, sequence:TTCACCTCTACTGTGTCAATGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20638 same embryo
	EMAGE:20639 same embryo
	EMAGE:20637 same embryo
	EurExpress:euxassay_013925 same experiment
	MGI:4826010 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source