EMAGE mouse gene expression spatial database

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EMAGE:20698

Srgap2 SLIT-ROBO Rho GTPase activating protein 2 ( MGI:109605)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013988. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013988_01	euxassay_013988_02	euxassay_013988_03	euxassay_013988_04

euxassay_013988_05	euxassay_013988_06	euxassay_013988_07	euxassay_013988_08	euxassay_013988_09

euxassay_013988_10	euxassay_013988_11	euxassay_013988_12	euxassay_013988_13	euxassay_013988_14

euxassay_013988_15	euxassay_013988_16	euxassay_013988_17	euxassay_013988_18	euxassay_013988_19

euxassay_013988_20	euxassay_013988_21	euxassay_013988_22	euxassay_013988_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20698_wholemount_strong.wlz

20698_wholemount_moderate.wlz

20698_wholemount_weak.wlz

20698_wholemount_possible.wlz

20698_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20698_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 15 16

thalamus mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 14 15 16

cerebral cortex mantle layer

strong

regional

strong expression: see section 18

cerebral cortex ventricular layer

strong

regional

strong expression: see section 04

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 11 12 13 15 16 17

midbrain mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 10 11 12 13 14 15 16

dorsal grey horn

strong

regional

strong expression: see section 05 06 07 09 10 11

heart ventricle

strong

spotted

strong expression: see section 14 15 moderate expression: see section 12 13 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39393
Entity Detected:	Srgap2, SLIT-ROBO Rho GTPase activating protein 2 ( MGI:109605)
Sequence:	sense strand is shown >T39393 GGCTCATGGAGATGTACAACAATGTTTTTTGCCCCCCTATGAAATTTGAATTCCAGCCCCACATGGGAGA TATGGCCTCTCAACTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTGCAGAGATGCCAACAGCTGCAG TCTCGCTTATCCACTTTGAAGATTGAGAATGAAGAGGTGAAAAAGACAATGGAGGCCACCCTGCAGACCA TTCAAGACATTGTGACTGTTGAGGATTTTGATGTATCTGACTGCTTCCAGTATAGCAATTCCATGGAGTC TGTCAAATCAACTGTCTCTGAAACATTCATGAGCAAGCCCAGCATTGCTAAGAGGAGAGCGAACCAGCAA GAGACGGAACAGTTTTATTTCACGCTTCAGCTCTCTAAGCTCCTTGTCTGTTCAGCAGCAACTGAACCCT TCAACCTCCACTCCCCTGATCAAGACAGGATTATTTATCCCCAGCATGTCTGTCCTGACTTCTGGAAAAT GAAGGAGTACTTGGAGGGTAGGAACCTCATCACAAAGCTACAAGCCAAGCATGACCTCCTGCAGAAAACC CTGGGAGAAAGTGAGTGTGAGCATGCTCTGCTGGAGGATTCCAGCCAAGCAATTCCTCTGGTGGTAGAAA GCTGCATCCGGTTTATTAGCAGACATGTGTACGTTATGAAGAAAAGAAATCAGGATGCAAGATGGGAAAT GGAGAGAGGCACTGTTGTGGTGGGTCATGAAAAGCCCACATTTTGGGTGGAGTTGTGGGGAAGAGGCCTA CAACATGAGGGAATTTTCCGGGTTTCTGGATCACAAGTAGAAGTGAACGACATAAAAAACGCCTTTGAGA GAGGAGAGGATCCCCTGGCTGGGGACCAGAATGACCATGACATGGACTCTATAGCTGGTGTCCTCAAGCT TTACTTCCGGGGCCTGGAACACCCGCTCTTCCCTAAAGACATCTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79588. Forward Primer - name:079588_F_cDNA_Fnbp2, sequence:GGCTCATGGAGATGTACAACAA; Reverse Primer - name:079588_N_SP6_cDNA_Fnbp2, sequence:GAAGATGTCTTTAGGGAAGAGCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20700 same embryo
	EMAGE:20697 same embryo
	EMAGE:20696 same embryo
	EMAGE:20699 same embryo
	EMAGE:20701 same embryo
	EurExpress:euxassay_013988 same experiment
	MGI:4828440 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source