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EMAGE:3303

En2 engrailed 2 ( MGI:95390)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3303
Figure 6M of Liu et al., 1999 [PMID:10518499] .Copyright: This image is from Liu A, Development 1999; 126:4827-38, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3303EMAGE:3303Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3303_voxel_strong_3D_1.wlz
3303_voxel_moderate_3D_1.wlz
3303_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3303_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
These results are shown schematically in Fig. 8 of this paper.
future hindbrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1346822
Entity Detected:En2, engrailed 2 ( MGI:95390)
Sequence:sense strand is shown

>MGI:1346822
AGATCTTTCTTTCATTTTTCTTTTCTCCCTTTTTTTAATCCCTTAAATTCCATTATGGGACATTGGACAC
TTCTTCCCCCGAAAGAAAAAAAATTTAAAACAACTTGCTGAAGTCCAAAGATTTTTATTGCTGCATTTCA
CAGGACTGTGAACCGAATAAATAGCTCCTATTTGGTCTGTGGGCTCTGCCGCTTGCTTTGTGCTGGCTCG
GACAGCACTGTAGGAAGCGCCAGCCAGAGTGGTGATGGAGAGCTCTGGTGGGGAGAGAGGGCGCAGTTCT
TTGCTGAGGTCTGAGAAAGTCCTTGCAGGCCAGCAGCTCTCCCGGAACCTCTCCGCCTGCTTCCTACGCA
GCCTCTCTTCTCAACCATCTCAGAGGCCCGTGCTGGCAGCCACCGTGTGTCTGCGTCTGTGTCGATGACC
CCTGTCCGCCACAGCCCCGTAGCGACCTGCCAGCGCCAACTCTCCCGCCACCGTGGCCAGCGTTGGGGCC
TGGGGAGCAGTGGGAGTGTGTCCTGAAGGCTTGAAGGCTTTGCTGCCGGGACAGCTAGCCCTGACCCAAA
AGCCCTCACCGAGATAGGCCTTGGGGCCTGGCAGAGCGAGTTGGAATCATCTTCCTTTCCTTTTTCTTCC
CTCTTTTTTCTTTTTCATTTATTTTTGAATGGTAAATACATGTATGTGTACATACTTGTGTATGCATGTG
CACGCACACCCCAGGCCTGTGTGAAGCTGGGGTTCTGGAGCTCACAGCTACCTTGACTTTCAAAGGAACT
CGAATCCTCCCGAAT
nt 2105 - nt 2889 of NM_010134.2
Notes:The probe used in this study, Liu et al., 1999 [PMID:10518499] , was originally described in Millen et al., 1995 [PMID:8575294] as "a 800 bp BglII-XbaI, 3' untranslated fragment". Editors Note: Digestion of the En2 mouse RefSeq mRNA sequence NM_010134.2 with BglII and XbaI identifies a 784nt 3'-UTR fragment (co-ordinates 2106-2889 as shown above).
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:Swiss Webster
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
General Information
Authors:Liu A; Losos K; Joyner AL, 1999 [PMID:10518499] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10518499] Liu A, Losos K, Joyner AL 1999 FGF8 can activate Gbx2 and transform regions of the rostral mouse brain into a hindbrain fate. Development (126):4827-38
 [ PMID:8575294] Millen KJ, Hui CC, Joyner AL 1995 A role for En-2 and other murine homologues of Drosophila segment polarity genes in regulating positional information in the developing cerebellum. Development (121):3935-45
Links:MGI:1349177 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI