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EMAGE:4324

Dsp desmoplakin ( MGI:109611)
TS18 (11 dpc)
in situ hybridisation

Data Images
EMAGE:4324 EMAGE:4324
Figure 3G. Copyright: This image is from Moore-Scott BA, Dev Dyn 2007 Jul;236(7):1997-2003 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright. Figure 3G'. Copyright: This image is from Moore-Scott BA, Dev Dyn 2007 Jul;236(7):1997-2003 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4324Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4324_wholemount_strong_3D_1.wlz
4324_wholemount_moderate_3D_1.wlz
4324_wholemount_possible_3D_1.wlz
4324_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4324_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
trunk somite
detected detected
heart
detected detected
tail somite
detected detected
hindgut
detected detected
regionalExpression is in the hindgut-cloaca endoderm.
anal region
detected detected
regionalExpression is in the hindgut-cloaca endoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3715930
Entity Detected:Dsp, desmoplakin ( MGI:109611)
Notes:The Dsp probe template used in this study by Moore-Scott et al., 2007 [PMID:17576135] is described by the authors as being generated by RT-PCR from isolated endoderm RNA using the following primers: "F: 5'-cca tag cct ttc tgt tcc att-3' 1361F; R: 5'-cct tat ctt cag cgt ctc ata-3' 2253R". Editors Note: The sequence of these primers cannot be found in various mouse Dsp mRNA sequences e.g. XM_621314.3, XM_896350.2, BC033467.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:11 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Moore-Scott BA; Opoka R; Lin SC; Kordich JJ; Wells JM, 2007 [PMID:17576135] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.21204] [ PMID:17576135] Moore-Scott BA, Opoka R, Lin SC, Kordich JJ, Wells JM 2007 Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation. Dev Dyn (236):1997-2003
Links:MGI:3716118 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI