Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4333

Hs6st2 heparan sulfate 6-O-sulfotransferase 2 ( MGI:1354959)
TS17 (10 dpc)
in situ hybridisation

Data Images
EMAGE:4333
Figure 7E. Copyright: This image is from Sedita J, Dev Dyn 2004 Dec;231(4):782-94 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Arrowheads indicate presence of signal.
Expression Pattern Description
Spatial Annotation:
EMAGE:4333Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4333_wholemount_strong_3D_1.wlz
4333_wholemount_moderate_3D_1.wlz
4333_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4333_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
regionalExpression is detected in the proximal forelimb mesenchyme in an anterior and posterior domain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3525283
Entity Detected:Hs6st2, heparan sulfate 6-O-sulfotransferase 2 ( MGI:1354959)
Sequence:sense strand is shown

>MGI:3525283
TCACTGAGTTTCAGCGCAAGACCCAGTACCTGTTTGAGAAGACCTTCAACATGAACTTTATCTCGCCGTT
TACCCAGTATAATACCACTAGGGCCTCTAGTGTTGAGATCAATGAGGAAATCCAAAAGCGTATTGAGGGA
CTGAATTTTCTGGATATGGAGTTGTACAGCTATGCTAAAGACCTCTTTCTGCAAAGGTATCAGTTCATGA
GGCAGAAAGAACATCAGGATGCCAGGCGGAAGCGTCAGGAGCAACGCAAATTTCTGAAGGGAAGGTTCCT
TCAGACCCATTTCCAGAGTCAGAGTCAGGGTCAGAGCCAGAGCCAGAGTCCAGGTCAGAATCTGAGTCAG
AATCCAAATCCTAACCCAAATCAGAACCTGACTCAGAACCTGAGTCACAATCTGACTCCGAGTTCAAATC
CCAATTCGACCCAGAGGGAGAACCGGGGAAGTCAGAAGCAGGGCTCAGGCCAGGGACAAGGTGATAGCGG
CACCAGCAATGGCACCAATGACTACATAGGGAGCGTAGAGACATGGCGCTAACTGGCACTCAAAGGCTTG
TGCAC
nt 939 - nt 1503 of NM_015819.3
Notes:The Hs6st2 (6OST2) probe used in this study by Sedita et al., 2004 [PMID:15499561] was generated by RT-PCR using the following primers: forward, 5'-TCACTGAGTTTCAGCGCAAG-3'; reverse, 5'-GTGCACAAGCCTTTGAGTGC-3'.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Sedita J; Izvolsky K; Cardoso WV, 2004 [PMID:15499561] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.20173] [ PMID:15499561] Sedita J, Izvolsky K, Cardoso WV 2004 Differential expression of heparan sulfate 6-O-sulfotransferase isoforms in the mouse embryo suggests distinctive roles during organogenesis. Dev Dyn (231):782-94
Links:MGI:3526062 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI