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EMAGE:4335

Hs6st3 heparan sulfate 6-O-sulfotransferase 3 ( MGI:1354960)
TS17 (10 dpc)
in situ hybridisation

Data Images
EMAGE:4335
Figure 7F. Copyright: This image is from Sedita J, Dev Dyn 2004 Dec;231(4):782-94 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Arrowhead indicates presence of signal.
Expression Pattern Description
Spatial Annotation:
EMAGE:4335Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4335_wholemount_strong_3D_1.wlz
4335_wholemount_moderate_3D_1.wlz
4335_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4335_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud ectoderm
detected detected
regionalExpression is detected in the distal epithelium of the forelimb
forelimb bud mesenchyme
detected detected
regionalExpression is detected in the distal mesenchyme of the forelimb
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3525284
Entity Detected:Hs6st3, heparan sulfate 6-O-sulfotransferase 3 ( MGI:1354960)
Sequence:sense strand is shown

>MGI:3525284
CAACAAGTGGCTGCTGACGCCGGTGCTCACTTTCCTCTTCGTGGTCATCATGTACCAGTACGTGTCCCCT
TCCTGCACCAGCTCATGCACCAACTTCGGGGAGCAGCTCCGCTCCGGGGAGGCCCGCCCGCCCGCTGTCC
CGAGTCCTGCCCGACGGGCACAAGCACCCCTTGATGAGTGGGAGCGGCGGCCCCAGCTGCCACCGCCACC
CCGGGGACCGCCCGAGGGGTCTCGTGGGGTCGCGGCTCCCGAGGATGAGGATGAGGATCCGGGGGACCCG
GAGGAGGAGGAGGAGGAAGAAGAGGAGGAGCCGGACCCCGAGGCGCCCGAAAACGGCTCCCTGCCCCGGT
TCGTGCCTCGATTCAACTTCACCCTCAAGGACCTGACCCGCTTCGTGGACTTCAACATCAAAGGGCGGGA
TGTGATCGTGTTCCTGCACATCCAGAAGACCGGGGGCACCACGTTCGGCCGGCACCTGGTGAAGAACATT
CGTCTGGAGCAGC
nt 128 - nt 630 of NM_015820.2
Notes:The Hs6st3 (6OST3) probe used in this study by Sedita et al., 2004 [PMID:15499561] was generated by RT-PCR using the following primers: forward, 5'-CAACAAGTGGCTGCTGACGCC-3'; reverse, 5'-GCTGCTCCAGACGAATGTTCTTCA-3'.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Sedita J; Izvolsky K; Cardoso WV, 2004 [PMID:15499561] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.20173] [ PMID:15499561] Sedita J, Izvolsky K, Cardoso WV 2004 Differential expression of heparan sulfate 6-O-sulfotransferase isoforms in the mouse embryo suggests distinctive roles during organogenesis. Dev Dyn (231):782-94
Links:MGI:3526064 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI