EMAGE mouse gene expression spatial database

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EMAGE:6091

Insrr insulin receptor-related receptor ( MGI:1346037)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011054. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011054_01	euxassay_011054_02	euxassay_011054_03	euxassay_011054_04

euxassay_011054_05	euxassay_011054_06	euxassay_011054_07	euxassay_011054_08	euxassay_011054_09

euxassay_011054_10	euxassay_011054_11	euxassay_011054_12	euxassay_011054_13	euxassay_011054_14

euxassay_011054_15	euxassay_011054_16	euxassay_011054_17	euxassay_011054_18	euxassay_011054_19

euxassay_011054_20	euxassay_011054_21	euxassay_011054_22	euxassay_011054_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6091_wholemount_strong.wlz

6091_wholemount_moderate.wlz

6091_wholemount_weak.wlz

6091_wholemount_possible.wlz

6091_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6091_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

single cell

strong expression: see section 10 11 12 13 14 18

glossopharyngeal ix ganglion

strong

single cell

strong expression: see section 08 09 20 21

trigeminal v ganglion

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 19 20 21 22 23

dorsal root ganglion

strong

single cell

strong expression: see section 09 10 11 12 13 14 17 18 19 20 21

medullary renal tubule

moderate

regional

moderate expression: see section 10 11 12 21 22 23

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36515
Entity Detected:	Insrr, insulin receptor-related receptor ( MGI:1346037)
Sequence:	sense strand is shown >T36515 GTGGAGTTCTAGAGGAGCTGGAGAACTGTCCGATTCAGCTACAAGAGCTGATGAGACTCTGCTGGCAGCA TAGTCCACGCCTGCGCCCGACTTTCGTCCACATCCTGGATCGCATACAGGATGAGCTTCGGCCCTCTTTC CGGCTCTGCTCCTTCTACTACAGCCCAGAGTGCCAGCGGGGTCAGGCCTCCTTGCTGCCTACTGAGGCAG AACCTGATTCCCCACCAACCTTAAATGGAGCTTCAGACTATAGTGCCCCAAATGGGGGCCCAGGACACTG ACGAGTCAACCTTTTCCCACCTGACTGGCCTCCCACGGGCAGAGAAGGCAGAACCCAACCTTTATGGCCA GCTGTTCCCCTCTCACCTCGAGTCCCCCTCAGACGAGTCAAGAATTAGCTCAGCACTCACAGAGATCAGG TGCCAGAAAGGGAGGCATCTGAACTCACAAATGCACAGACTCACAGAGACAGCGTGTGGGAGATCATGAG AGGCTGCAGCAGGAGGGACTCTCAGGCCATAGGGCAGAAGTGCTCTGCCCTCAGAGAGAAACACTGGTCA GACTGAGAAAGCTAGCTCATGGGAGGCAGGGTCCAGAGACACACGCCTCCAACTTCAGGTGGTTAACAGC TTGAAAGCAAATGAGTGGCCAGAGACAGGCCTGGGAGGTCTCTCCACTCATGTTGGGGCCTGGGGACAAG GATTCCTTCTTTCCCTGTGGTTGGCTCCTCCCCCTCTGTCCCACATACCAGGACAAAGCAGGAACCCTAG GACCCTCACTCCTCTCCCCCTGCTCTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74592. Forward Primer - name:074592_F_cDNA_Insrr, sequence:GTGGAGTTCTAGAGGAGCTGGA; Reverse Primer - name:074592_N_SP6_cDNA_Insrr, sequence:GTAGAGCAGGGGGAGAGGAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4825605 same experiment
	EurExpress:euxassay_011054 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source