EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6249

Ralgapa2 Ral GTPase activating protein, alpha subunit 2 (catalytic) ( MGI:3036245)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016072. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016072_01	euxassay_016072_02	euxassay_016072_03	euxassay_016072_04

euxassay_016072_05	euxassay_016072_06	euxassay_016072_07	euxassay_016072_08	euxassay_016072_09

euxassay_016072_10	euxassay_016072_11	euxassay_016072_12	euxassay_016072_13	euxassay_016072_14

euxassay_016072_15	euxassay_016072_16	euxassay_016072_17	euxassay_016072_18	euxassay_016072_19

euxassay_016072_20	euxassay_016072_21	euxassay_016072_22	euxassay_016072_23	euxassay_016072_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6249_wholemount_strong.wlz

6249_wholemount_moderate.wlz

6249_wholemount_weak.wlz

6249_wholemount_possible.wlz

6249_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6249_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 14

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 09 10 11 14 16 weak expression: see section 15

midbrain ventricular layer

moderate

regional

moderate expression: see section 10 11 16 17 18 weak expression: see section 12 13

cochlea

moderate

regional

moderate expression: see section 07 08 09 10 18 19 20 21 weak expression: see section 17

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39835
Entity Detected:	Ralgapa2, Ral GTPase activating protein, alpha subunit 2 (catalytic) ( MGI:3036245)
Sequence:	sense strand is shown >T39835 CGTGGAAGAGGAAAAGAAGAGAAGAAGTGTGGAATTGATCCCCCTCACTGCACGAATGGTCATGGCCCAC CTAGTGAACCACCTAGGCCACTATCCACTGAGTGGGGGTCCTGCTGTGCTGCACAGCCTGGTCAGTGAGA ACCATGACAATGCCCATGTGGAAGGAACTGAGCTGTCCTCGGAGGTGTTCAGGAGCCCAAACCTGCAGCT CTTCGTATTTAATGATAGCACCCTCATCTCCTATCTTCAGACACCAGCAGAAGGACCAGCTGGAGGGACT TCAGGGGGTTCCCTCTCAGATGTGAGAGTCATTGTGAGGGATATCTCAGGGAAGTACTCTTGGGATGGCA AGGTTTTATATGGACCTCTGGAAGGCCGTTTGGCACCCAATGGAAGGAACCCCTCATTTCAGATTTCTGG CTGGCATCATCACACATGTGGACCTCAGAAAGATTTGTTTAACGGTGAGGAGGGAGATGATGTGCTTGAC AAGTTACTTGAAAACATTGGCCATACAAGCCCCGAATGCCTTTTACCATCACAACTAAATCTAAATGAGC CTTCCCCAACCCCATGTGCAATGAACTGGGACCAAGAGAAGGCAATCATGGAGGTCATCCTGCGCCAGAG CGCACAGGAAGATGAGTATGTGCAGAGGTGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79763. Forward Primer - name:079763_F_cDNA_BC053994, sequence:CGTGGAAGAGGAAAAGAAGAGA; Reverse Primer - name:079763_N_SP6_cDNA_BC053994, sequence:ACACCTCTGCACATACTCATCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6250 same embryo
	EMAGE:6248 same embryo
	EMAGE:6252 same embryo
	EMAGE:6251 same embryo
	EMAGE:6253 same embryo
	EurExpress:euxassay_016072 same experiment
	MGI:4827597 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source