EMAGE mouse gene expression spatial database

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EMAGE:6307

Gramd4 GRAM domain containing 4 ( MGI:2676308)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007287. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007287_01	euxassay_007287_02	euxassay_007287_03	euxassay_007287_04

euxassay_007287_05	euxassay_007287_06	euxassay_007287_07	euxassay_007287_08	euxassay_007287_09

euxassay_007287_10	euxassay_007287_11	euxassay_007287_12	euxassay_007287_13	euxassay_007287_14

euxassay_007287_15	euxassay_007287_16	euxassay_007287_17	euxassay_007287_18	euxassay_007287_19

euxassay_007287_20	euxassay_007287_21	euxassay_007287_22	euxassay_007287_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6307_wholemount_strong.wlz

6307_wholemount_moderate.wlz

6307_wholemount_weak.wlz

6307_wholemount_possible.wlz

6307_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6307_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 17 18

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2071
Entity Detected:	Gramd4, GRAM domain containing 4 ( MGI:2676308)
Sequence:	sense strand is shown >T2071 TGGCCTCGAGCCAGATTCGGCACGAGGAACGCTCGTTTGCCGGCGTAGGTGTGAGGCCCGGTGGCTTGGG CTCATGACACCTCATTCCCAGGGGACTTCAGGCCACACACTGGGGGTGGGAAGGGCTTGCCAGGCAACCA GATCAAGCACACATACCACTCCAGATGCGTAGCAGAGGCCACACAGCTCCAGGTACATAGACGGAGGAGT CTGGATGGGGAGGCGGGACCCTCGACCCAGGGCCGTGGCTTGGGCCCCTGGAATATGCTTGTGCTATGAA GAGCATGGAATCAGAGAAGGAGCACCCCAACGTGCCAGAGGGAAGGCAACATGGCCTCTGACCTGTCCGT GAGGATGGCTTCATGGGTGATAGCCACACATGGTGGCTAACACTTCATGGACCCTGGCGGTGCCTGGGTT GTGAGTGTTGCCACCCCGGTGTGCTACAGAGAGGCCTTAAGCAGAGAAGGCTTTCAGGGTGGAATACGTG GTTTAGTTTGAGTCTCTTTCTTTGGGAAAGAGAGATGAAGACTGACCATGGGTCA
Notes:	The probe template was PCR amplified from IMAGE:831760 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:831760 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6309 same embryo
	EMAGE:6308 same embryo
	EMAGE:6306 same embryo
	EurExpress:euxassay_007287 same experiment
	MGI:4825213 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source