EMAGE mouse gene expression spatial database

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EMAGE:6724

Ttc39c tetratricopeptide repeat domain 39C ( MGI:1919997)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007378. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007378_01	euxassay_007378_02	euxassay_007378_03	euxassay_007378_04

euxassay_007378_05	euxassay_007378_06	euxassay_007378_07	euxassay_007378_08	euxassay_007378_09

euxassay_007378_10	euxassay_007378_11	euxassay_007378_12	euxassay_007378_13	euxassay_007378_14

euxassay_007378_15	euxassay_007378_16	euxassay_007378_17	euxassay_007378_18	euxassay_007378_19

euxassay_007378_20	euxassay_007378_21	euxassay_007378_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6724_wholemount_strong.wlz

6724_wholemount_moderate.wlz

6724_wholemount_weak.wlz

6724_wholemount_possible.wlz

6724_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6724_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

regional

strong expression: see section 05 06 07 14 15 16

brain

strong

regional

strong expression: see section 09 moderate expression: see section 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 15 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 07 14

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 14 15 16 17 18

anterior naris

strong

regional

strong expression: see section 06 07 09 10

external naris

strong

regional

strong expression: see section 06 10 11

seminiferous cord

moderate

regional

moderate expression: see section 05 06 07 15 16 17 18

clavicle

strong

regional

strong expression: see section 09 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3307
Entity Detected:	Ttc39c, tetratricopeptide repeat domain 39C ( MGI:1919997)
Sequence:	sense strand is shown >T3307 TGGCCTCGAGGCCAGATTCGGACGAGGCGGAAATCCCCCCCTCCATGGTCGATCGACTTCAGAGGCAGAT CATCATCGCTGACTGCCAGGTTTACCTGGCAGTGCTTTCCTTTGTGAAACAAGAGTTGTCGGCCTACATA AAAGGTGGGTGGATCCTTAGGAAAGCCTGGAAGATTTACAGTAAGTGCTATGTGGACATCAACGCCCTTC AGGAACTGTATCAGAAGAAGCTGACGGAAGAGCCCTTGGCTTCTGATGCTGCAAATGATAACCACGTTGT GGCCGAAGGGGTGACCGAGGAGTCCTTAAGCAGACTGAAAGGGGCTGTGAGCTTTGGATATGGCCTTTTC CACCTTTGCATATCCATGGTGCCCCCAAACCTGCTCAAAATCATCAACCTGCTGGGCTTTCCTGGAGACC GCCTACAGGGCCTTTCTTCACTGACGTATGCGAGCGAAAGTAAGGACATGAAGGCCCCTTTAGCTACATT AGCCCTGCTGTGGTACCACACTGTGGTCCGCCCATTTTTTGCTTTGG
Notes:	The probe template was PCR amplified from IMAGE:2780732 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2780732 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6722 same embryo
	EMAGE:6720 same embryo
	EMAGE:6721 same embryo
	EMAGE:6719 same embryo
	EMAGE:6723 same embryo
	EurExpress:euxassay_007378 same experiment
	MGI:4828990 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source