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EMAGE:7830

Scn11a sodium channel, voltage-gated, type XI, alpha ( MGI:1345149)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7830 EMAGE:7830 EMAGE:7830 EMAGE:7830 EMAGE:7830
"Pseudo-wholemount" of euxassay_007407. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007407_01 euxassay_007407_02 euxassay_007407_03 euxassay_007407_04
EMAGE:7830 EMAGE:7830 EMAGE:7830 EMAGE:7830 EMAGE:7830
euxassay_007407_05 euxassay_007407_06 euxassay_007407_07 euxassay_007407_08 euxassay_007407_09
EMAGE:7830 EMAGE:7830 EMAGE:7830 EMAGE:7830 EMAGE:7830
euxassay_007407_10 euxassay_007407_11 euxassay_007407_12 euxassay_007407_13 euxassay_007407_14
EMAGE:7830 EMAGE:7830 EMAGE:7830 EMAGE:7830 EMAGE:7830
euxassay_007407_15 euxassay_007407_16 euxassay_007407_17 euxassay_007407_18 euxassay_007407_19
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euxassay_007407_20 euxassay_007407_21 euxassay_007407_22 euxassay_007407_23 euxassay_007407_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7830Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7830_wholemount_strong.wlz
7830_wholemount_moderate.wlz
7830_wholemount_weak.wlz
7830_wholemount_possible.wlz
7830_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7830_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trigeminal v ganglion
moderate moderate
single cellmoderate expression: see section 03 04 05 17 18 21 22 weak expression: see section 06 07 19 20
dorsal root ganglion
moderate moderate
single cellmoderate expression: see section 07 08 09 10 15 17 18 weak expression: see section 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35293
Entity Detected:Scn11a, sodium channel, voltage-gated, type XI, alpha ( MGI:1345149)
Sequence:sense strand is shown

>T35293
GGATGTTCTCTTTGCTTTCACCACCAGGGTCCTCGGGAACTCCAGCGGCTTGGATACCATGAAAGCCATG
ATGGAGGAGAAGTTCATGGAGGCTAATCCTTTCAAGAAGTTGTACGAGCCCATTGTCACCACCACAAAGA
GGAAGGAGGAGGAGGAATGTGCCGCTGTCATCCAGAGGGCCTACCGGAGACACATGGAGAAGATGATCAA
GCTGAAGCTGAAAGGCAGGTCAAGTTCATCGCTCCAGGTGTTTTGCAATGGAGACTTGTCTAGCTTGGAT
GTGCCCAAGATCAAGGTTCATTGTGACTGAAACCCCCACCTGCACGCCTACCTCACAGCCTCACAGCTCA
GCCCCCAGCCTCTGGCGAACAAGCGGCGGACTCACCGAACAGGCCGTTCAACTTGTTTTTTTGGGTGAAA
GAGGTGATAGGTTGGTGTCCATTTTTAAATGATTCTTGGAAAGATTGAACGTCGGAACATGTTAGAAAGG
ACTGCCAAGGACATCCACAGTAACGGAAGGCCTGAAGGACAGTTCAAATTATGTAAAGAAACGAGAAGGA
AAGGTCACATGTCTGTTCAGTTTTAAGTATGTGACCTGCCACATGTAGCTCCTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95094. Forward Primer - name:095094_F_cDNA_Scn11a, sequence:GGATGTTCTCTTTGCTTTCACC; Reverse Primer - name:095094_N_SP6_cDNA_Scn11a, sequence:AAAGGAGCTACATGTGGCAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7829 same embryo
 EMAGE:7828 same embryo
 EMAGE:7827 same embryo
 EurExpress:euxassay_007407 same experiment
 MGI:4827901 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS