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EMAGE:7952

Dock8 dedicator of cytokinesis 8 ( MGI:1921396)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7952 EMAGE:7952 EMAGE:7952 EMAGE:7952 EMAGE:7952
"Pseudo-wholemount" of euxassay_013103. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013103_01 euxassay_013103_02 euxassay_013103_03 euxassay_013103_04
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View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7952Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7952_wholemount_strong.wlz
7952_wholemount_moderate.wlz
7952_wholemount_weak.wlz
7952_wholemount_possible.wlz
7952_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7952_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 10 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38672
Entity Detected:Dock8, dedicator of cytokinesis 8 ( MGI:1921396)
Sequence:sense strand is shown

>T38672
TGGATCCTAACAAGGCCTACATTCAGATCACTTTTGTGGAGCCTTACTTTGATGAATATGAGATGAAAGA
CCGGGTGACCTACTTCGAGAAGAATTTCAACCTCCGGAGGTTCATGTACACCACCCCCTTCACCCTGGAG
GGGAGACCCCGGGGCGAGCTTCATGAGCAACACCGCAGAAACACCGTGCTCACCACCATGCACGCCTTCC
CCTACATCAAGACCAGGATCCGAGTCAGCCAGAAAGAGGAGTTCGTTTTGACTCCGATTGAGGTTGCCAT
TGAAGATATGAAGAAGAAGACCCTGCAGTTAGCCGTGGCCACTCACCAGGAGCCCCCTGATGCAAAGATG
CTGCAAATGGTACTGCAGGGCTCTGTAGGAGCCACTGTAAATCAGGGACCACTGGAGGTGGCCCAAGTGT
TCTTGGCTGAAATTCCAGCTGACCCAAAGCTCTACCGACATCACAACAAGCTGAGGTTGTGCTTCAAGGA
GTTCATAATGCGATGCGGAGAGGCCGTGGAGAAGAACAGGCGACTCATCACCGCAGAGCAGCGGGAGTAC
CAGCAGGAGCTGAAGAAGAACTACAACAAGCTGAGAGACAGCCTCAGGCCCATGATTGAGCGGAAAATCC
CAGAGCTCTACAAGCCCATATTCAGAGTTGACAGTCAGAAGAGGGACTCTTTCCACAGATCTAGTTTCAG
GAAATGTGAAACCCAGTTGTCACAGGGCAGCTGAAAAAAGCCACCTTTGCCGGTGACGACTGGGGCCCCA
CTACCTGGAAGGACTCGCTGGTACGTCCACAGCAGCCACCCAAGACTGACTCCACAGTCCCTGCCCAGCC
AGTGCTCTGGAAACTTGGGGGGTCTATGACCACAGAATGATACCCAAGTGTATTGTGGACTATCTTGACC
ATGTCAGGGGTTGGGGTGGGGTGGGTAAATCAGGGGATAATTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 154882. Forward Primer - name:154882_F_cDNA_TC1562340, sequence:TGGATCCTAACAAGGCCTACAT; Reverse Primer - name:154882_N_SP6_cDNA_TC1562340, sequence:CAATTATCCCCTGATTTACCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7951 same embryo
 EMAGE:7950 same embryo
 EurExpress:euxassay_013103 same experiment
 MGI:4824372 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS