EMAGE mouse gene expression spatial database

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EMAGE:8306

Npr1 natriuretic peptide receptor 1 ( MGI:97371)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007624. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007624_01	euxassay_007624_02	euxassay_007624_03	euxassay_007624_04

euxassay_007624_05	euxassay_007624_06	euxassay_007624_07	euxassay_007624_08	euxassay_007624_09

euxassay_007624_10	euxassay_007624_11	euxassay_007624_12	euxassay_007624_13	euxassay_007624_14

euxassay_007624_15	euxassay_007624_16	euxassay_007624_17	euxassay_007624_18	euxassay_007624_19

euxassay_007624_20	euxassay_007624_21	euxassay_007624_22	euxassay_007624_23	euxassay_007624_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8306_wholemount_strong.wlz

8306_wholemount_moderate.wlz

8306_wholemount_weak.wlz

8306_wholemount_possible.wlz

8306_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8306_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

adrenal gland

strong

regional

strong expression: see section 04 05 06 07 13 14 15 not examined expression: see section 12

thyroid gland

weak

regional

weak expression: see section 10 11 15 16

bladder

moderate

regional

moderate expression: see section 11 12 13

renal cortex

moderate

regional

moderate expression: see section 04 05 06 07 weak expression: see section 08 14 15 16 17 18

testis

strong

regional

strong expression: see section 03 04 05 06 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36698
Entity Detected:	Npr1, natriuretic peptide receptor 1 ( MGI:97371)
Sequence:	sense strand is shown >T36698 TTTCGGTACTCACTCACCAATGACATTGTCAAGGGAATGCTCTTTCTACACAACGGGGCCATTTGTTCCC ATGGGAACCTCAAGTCATCCAACTGCGTGGTAGATGGACGTTTTGTGTTAAAGATCACAGACTATGGGCT CGAGAGCTTCAGAGACCCGGAGCCAGAGCAAGGACACACCCTCTTTGCCAAAAAACTGTGGACTGCACCT GAGCTCCTGCGAATGGCTTCCCCACCTGCCCGTGGCTCCCAAGCTGGGGATGTCTACAGTTTTGGTATCA TCCTTCAGGAAATTGCCCTAAGAAGTGGGGTCTTCTATGTGGAAGGTTTGGACCTCAGCCCAAAAGAGAT CATTGAGCGTGTGACTCGGGGTGAGCAGCCCCCATTCCGACCTTCCATGGATCTGCAGAGCCACCTGGAG GAACTGGGGCAGCTGATGCAGAGGTGCTGGGCAGAGGATCCTCAGGAGCGGCCACCCTTTCAACAGATCC GCCTGGCGCTGCGCAAGTTCAACAAGGAGAACAGCAGCAACATCCTGGACAACCTGCTGTCACGCATGGA ACAGTACGCCAACAACCTGGAGGAACTGGTAGAGGAGAGAACACAGCCTTATCTGGAGGAGAAGCGCAAA GCTGAGGCCCTGCTTTACCAGATTCTGCCTCACTCTGTGGCTGAGCAGCTGAAGAGAGGCGAGACAGTCC AGGCTGAGGCATTTGATAGTGTTACTATCTATTTCAGTGATATCGTGGGCTTTACAGCTCTTTCAGCAGA GAGCACACCCATGCAGGTGGTCACCCTGCTCAATGATCTGTACACCTGTTTTGATGCTGTCATAGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96004. Forward Primer - name:096004_F_cDNA_Npr1, sequence:TTTCGGTACTCACTCACCAATG; Reverse Primer - name:096004_N_SP6_cDNA_Npr1, sequence:GTCTATGACAGCATCAAAACAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8304 same embryo
	EMAGE:8303 same embryo
	EMAGE:8305 same embryo
	EMAGE:8302 same embryo
	EMAGE:8301 same embryo
	EurExpress:euxassay_007624 same experiment
	MGI:4826770 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source