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EMAGE:8764

Srbd1 S1 RNA binding domain 1 ( MGI:1925836)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8764 EMAGE:8764 EMAGE:8764 EMAGE:8764 EMAGE:8764
"Pseudo-wholemount" of euxassay_009989. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009989_01 euxassay_009989_02 euxassay_009989_03 euxassay_009989_04
EMAGE:8764 EMAGE:8764 EMAGE:8764 EMAGE:8764 EMAGE:8764
euxassay_009989_05 euxassay_009989_06 euxassay_009989_07 euxassay_009989_08 euxassay_009989_09
EMAGE:8764 EMAGE:8764 EMAGE:8764 EMAGE:8764 EMAGE:8764
euxassay_009989_10 euxassay_009989_11 euxassay_009989_12 euxassay_009989_13 euxassay_009989_14
EMAGE:8764 EMAGE:8764 EMAGE:8764 EMAGE:8764 EMAGE:8764
euxassay_009989_15 euxassay_009989_16 euxassay_009989_17 euxassay_009989_18 euxassay_009989_19
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View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8764Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8764_wholemount_strong.wlz
8764_wholemount_moderate.wlz
8764_wholemount_weak.wlz
8764_wholemount_possible.wlz
8764_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8764_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 11 12 13 14 15
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 17 18 weak expression: see section 10 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31138
Entity Detected:Srbd1, S1 RNA binding domain 1 ( MGI:1925836)
Sequence:sense strand is shown

>T31138
TGCAGGACTCAATGCCAATAGAGCCAAAAATATTATTGAGTGGCGAGAGAAAAATGGCCCTTTCATTAAC
CGAGAACAGTTGAAGAAGGTGAAAGGGCTTGGTCCAAAATCCTTCCAGCAGTGTGCTGGCTTCGTCAGGA
TCAACCAGGATTACATCCGGACATTCTGCAGTCAACACACTGACTCTTCAGGCCAGAGCCAGGAAACTGC
CATGGTCACAAATGAGAAGCTGGGCAAAAAGAAGAACAAAGCAGATGCCACCCTCATACCAAACCCACTG
GACCAGACTTGCATCCATCCGGAATCCTATGACATAGCAGTCAGGTTTTTATCGTTCATTGGGGGGACAA
TGTGTGAGATTGGAAAGCCTGAAATGCAGCAGAAAATAAACGTGTCTCTCGGGAAAGAAGGCATAGAGGA
AACTGCTGAAAGGTTACAGACAACTGTGCACACACTGCAGGTCATCATAGATGGACTGAGCCAGCCCAAA
ACCTTTGACATCCGAACAGATTTTGATAAGCCTGACTTCAAGAGAAGCATAGTGTGTCTGGAAGACCTGC
AGGTTGGGACAGTTCTTACAGGCAAAGTGGAGAATGCTACTCTGTTTGGGGTCTTTGTGGATATAGGAGT
GGGGAAGGCGGGGCTGATTCCTATCCGGTTCATAACAGAAGCCAAGCTGTCCAAAACAAAGAGGAGAAGA
AGCCTGGGCCTGGGCCCCGGAGAGAAAGTGGAGGTGAAAGTCCTCAATGTCGACATTCCCCGATCTCGGA
TTTCGCTCGACCTCCTCCGGGTGTTGTGAGTGCCCCACCGAAGGCCAAACACCAATGTGTTCCCTCACTT
CTACAGCCTGACAAGGACGAGCCAGAGCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:1384147), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57441. Forward Primer - name:057441_F_IRAV43_h12_AI461933, sequence:TGCAGGACTCAATGCCAA; Reverse Primer - name:057441_R_SP6_IRAV43_h12_AI461933, sequence:TGAGCTCTGGCTCGTCCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8769 same embryo
 EMAGE:8766 same embryo
 EMAGE:8767 same embryo
 EMAGE:8765 same embryo
 EMAGE:8768 same embryo
 EurExpress:euxassay_009989 same experiment
 MGI:4828435 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS