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EMAGE:8905

Klhl9 kelch-like 9 (Drosophila) ( MGI:2180122)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905
"Pseudo-wholemount" of euxassay_012859. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012859_01 euxassay_012859_02 euxassay_012859_03 euxassay_012859_04
EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905
euxassay_012859_05 euxassay_012859_06 euxassay_012859_07 euxassay_012859_08 euxassay_012859_09
EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905
euxassay_012859_10 euxassay_012859_11 euxassay_012859_12 euxassay_012859_13 euxassay_012859_14
EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905
euxassay_012859_15 euxassay_012859_16 euxassay_012859_17 euxassay_012859_18 euxassay_012859_19
EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905 EMAGE:8905
euxassay_012859_20 euxassay_012859_21 euxassay_012859_22 euxassay_012859_23 euxassay_012859_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8905Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8905_wholemount_strong.wlz
8905_wholemount_moderate.wlz
8905_wholemount_weak.wlz
8905_wholemount_possible.wlz
8905_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8905_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 10 11 17 18
pons mantle layer
weak weak
regionalweak expression: see section 09 19
ventral grey horn
weak weak
regionalweak expression: see section 12 13 14 16 17
neural retina
weak weak
regionalweak expression: see section 01 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38480
Entity Detected:Klhl9, kelch-like 9 (Drosophila) ( MGI:2180122)
Sequence:sense strand is shown

>T38480
CACTGATGACACCACAGGATCTCATCAACTATGTGCAGACAGTAGATTTCATGAGAACAGACAATACCTG
TGTGAATTTACTTTTGGAAGCAAGCAATTACCAAATGATGCCGTATATGCAGCCGGTGATGCAGTCGGAC
AGAACTGCCATTCGATCTGATTCTACTCATTTGGTTACCTTAGGAGGAGTTTTGAGACAACAGCTAGTTG
TCAGTAAAGAGTTACGAATGTACGACGAAAGGGCGCAGGAGTGGAAGTCGTTAGCGCCCATGGATGCTCC
TCGATACCAGCATGGCATTGCTGTTATTGGAAATTTCCTTTATGTAGTTGGTGGCCAGAGTAATTATGAT
ACAAAAGGAAAAACGGCTGTTGATACAGTTTTCAGATTTGATCCTCGTTATAATAAGTGGATGCAGGTGG
CATCATTAAACGAAAAGCGCACATTCTTTCACCTGAGTGCCCTCAAAGGACATTTATATGCAGTTGGTGG
GCGCAGTGCAGCTGGTGAACTGGCCACAGTAGAATGTTACAACCCAAGAATGAATGAATGGAGCTACGTT
GCAAAGATGAGTGAACCCCACTATGGCCATGCTGGAACAGTATATGGGGGTTTAATGTATATTTCCGGAG
GAATCACTCATGACACTTTCCAAAATGAGCTCATGTGTTTTGACCCAGATA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150921. Forward Primer - name:150921_F_cDNA_C530050O22Rik, sequence:CACTGATGACACCACAGGATCT; Reverse Primer - name:150921_N_SP6_cDNA_C530050O22Rik, sequence:TATCTGGGTCAAAACACATGAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8904 same embryo
 EMAGE:8903 same embryo
 EurExpress:euxassay_012859 same experiment
 MGI:4825809 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS