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EMAGE:8935

Krt32 keratin 32 ( MGI:1309995)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935
"Pseudo-wholemount" of euxassay_012871. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012871_01 euxassay_012871_02 euxassay_012871_03 euxassay_012871_04
EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935
euxassay_012871_05 euxassay_012871_06 euxassay_012871_07 euxassay_012871_08 euxassay_012871_09
EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935
euxassay_012871_10 euxassay_012871_11 euxassay_012871_12 euxassay_012871_13 euxassay_012871_14
EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935
euxassay_012871_15 euxassay_012871_16 euxassay_012871_17 euxassay_012871_18 euxassay_012871_19
EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935 EMAGE:8935
euxassay_012871_20 euxassay_012871_21 euxassay_012871_22 euxassay_012871_23 euxassay_012871_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8935Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8935_wholemount_strong.wlz
8935_wholemount_moderate.wlz
8935_wholemount_weak.wlz
8935_wholemount_possible.wlz
8935_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8935_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
esophagus
weak weak
regionalweak expression: see section 12
mandible
weak weak
regionalweak expression: see section 04 05 06 07 08 09 12 13 16 17 18 19
maxilla
weak weak
regionalweak expression: see section 05 19
urethra of male
weak weak
regionalweak expression: see section 13
lung
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 14 15 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38524
Entity Detected:Krt32, keratin 32 ( MGI:1309995)
Sequence:sense strand is shown

>T38524
GAGGCTCAAGTAGAGTCCCTGAAGGAGGAACTTCTGTGCCTCAAGAGGAACCATGAAGAGGAAGTTGGTG
TCCTTCGACAACAGCTCGGGGACCGCCTTAACATTGAGGTAGATGCTGCACCTCCCGTGGACCTAACCAG
GATGCTGGAGGAGGTGAGATGTCAGTATGAGACCATGGTGGAAACCAACCACAGGGACGTGGAGGAATGG
TTCAATATGCAGATGGAGGAGCTTAACAAGCAGGTGGCCACAAGCTCTGAGCAGCTTCAGAGCTACCAAT
CAGACATCATTGACCTGAGACGAACAGTCAATACTCTGGAGATAGAACTGCAGGCTCAGCACAGCCTGAG
AGACTCCCTGGAAAACACGCTGGGGGAGACAGAGGGCCGCTTCACCTCCCAGCTGTCCCAGATGCAATGC
ATGATCACCAATGTGGAGTCCCAGCTGTCTGACATCCGCTGTGACCTGGAGAGACAGAACCAAGAGTACA
AGGTGTTGCTGGATGTCAAGGCCCGGCTGGAGTGTGAGATTGACACGTACAGGGGCCTGCTGGAGAGTGA
GGACAGCAAGCTACCCTGTAACCCTTGCTCCACTCCCTCCTGCCAGCCTTGTGCACCCTCTCCTGGGGTA
TCCCGCACCGTCTGTGTGCCCCACACTGTTTGTGTGCCTTGTTCACCCTGCCTACAGACCCGCTACTGAA
GCCTCTGAGCATCTCTCAGTGTGTGAGTCCCAGAGGGACCTGAGTTCTCTGGCCTGGTGCCTCGTCACAC
CAAGTCTGACCACAATCCTGAGACCAAGACAAGAGTGTGGCCAGCTTAGTTGGCCCTGACCAGAACCAGG
AAGTGACACTAGCAACATGTCCTGATAATACATTGCTTCAGCGCTGCCTCCTCTATTTCCTTGGGTAGAA
GGTTCTCTTTGGTGGTGTTTCTGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150731. Forward Primer - name:150731_F_cDNA_Krt1-2, sequence:GAGGCTCAAGTAGAGTCCCTGA; Reverse Primer - name:150731_N_SP6_cDNA_Krt1-2, sequence:CCAGAAACACCACCAAAGAGAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8937 same embryo
 EMAGE:8938 same embryo
 EMAGE:8936 same embryo
 EMAGE:8934 same embryo
 EMAGE:8939 same embryo
 EurExpress:euxassay_012871 same experiment
 MGI:4825838 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS