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EMAGE:9250

Foxd3 forkhead box D3 ( MGI:1347473)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250
"Pseudo-wholemount" of euxassay_019647. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019647_01 euxassay_019647_02 euxassay_019647_03 euxassay_019647_04
EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250
euxassay_019647_05 euxassay_019647_06 euxassay_019647_07 euxassay_019647_08 euxassay_019647_09
EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250
euxassay_019647_10 euxassay_019647_11 euxassay_019647_12 euxassay_019647_13 euxassay_019647_14
EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250
euxassay_019647_15 euxassay_019647_16 euxassay_019647_17 euxassay_019647_18 euxassay_019647_19
EMAGE:9250 EMAGE:9250 EMAGE:9250 EMAGE:9250
euxassay_019647_20 euxassay_019647_21 euxassay_019647_22 euxassay_019647_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9250Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9250_wholemount_strong.wlz
9250_wholemount_moderate.wlz
9250_wholemount_weak.wlz
9250_wholemount_possible.wlz
9250_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9250_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 11 12
pons mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 13 weak expression: see section 12 14
ventral grey horn
moderate moderate
regionalmoderate expression: see section 07 09 10 weak expression: see section 08 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55051
Entity Detected:Foxd3, forkhead box D3 ( MGI:1347473)
Sequence:sense strand is shown

>T55051
CGCTGTCTGGACAGTTCCTGCAGCCCGCAGCCTCAGCCGCAGCGGCTGCGGCCGCCGCCGTGCAAGCCAA
GTGGCCCGCTCAATAGTGATGAGCTAGTGGCCGGGCCGGACGGCGGCCTCTGGCAGCAGCCTGCTCTTCC
AGGCGGTACGCCCTCTCTGATCCTGGTCCATCTGTCCTGGTCCTGGTCCTGGTCCCTCTACCCAATCCTG
GACTCTGCTACCCGCCCCCGACTCCCCAACACTGACCAACAGCGGCCTCCATCCCCTCACTCACCTAAGC
TGGCGGAGCAAACTCAACCCGTCCGCTGGGAATAACTTTCCAGTACAGGTAAAACTGATAACCGAATTTC
CAAAAATGCACCCCGACGGCGCCGTTGTTCTCAGTACTGTGGGGGTGGGAGGGACATTCTTTGTACATAT
TTGTATAAAAATCATTGACTTTCCTTTTGGGGTTTTTATTTTTTTAAGAAAATAAAAAGCAATTCAGTAG
ATTTAGAGCTTTGAACTTTCATATTTTTTTGAAGGTTCACTCTGCGCAGTTTTATCTGAGAAAAGAATGT
ATAAAGACTTGGGAGATTTAAATACAAAAAATTTTTCAAAAAGGCGAAGTGTCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CGCTGTCTGGACAGTTCCT; Reverse Primer - name:unspecified, sequence:TGACACTTCGCCTTTTTGAA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9253 same embryo
 EMAGE:9251 same embryo
 EMAGE:9252 same embryo
 EurExpress:euxassay_019647 same experiment
 MGI:4824902 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS