Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3949

Wnt1 wingless-related MMTV integration site 1 ( MGI:98953)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:3949 EMAGE:3949 EMAGE:3949 EMAGE:3949 EMAGE:3949
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: no stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Spatial Annotation:
EMAGE:3949Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3949_voxel_strong_3D_1.wlz
3949_voxel_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3949_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: no stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt1 probeA
Entity Detected:Wnt1, wingless-related MMTV integration site 1 ( MGI:98953)
Sequence:sense strand is shown

>Wnt1 probeA
AGTGGCTGCTTCAGCCCAGCAGCCAGGACAGCGAACCATGCTGCCTGCGGCCCGCCTCCAGACTTATTAG
AGCCAGCCTGGGAACTCGCATCACTGCCCTCACCGCTGTGTCCAGTCCCACCGTCGCGGACAGCAACCAC
AGTCGTCAGAACCCCAGCACAGAACCAGCAAGGCCAGGCAGGCCATGGGGCTCTGGGCGCTGCTGCCCAG
CTGGGTTTCTACTACGTTGCTACTGGCACTGACCGCTCTGCCCGCAGCCCTAGCTGCCAACAGTAGTGGC
CGATGGTGGGGCATCGTGAACATAGCCTCCTCCACGAACCTGTTGACGGATTCCAAGAGCCTGCAGCTGG
TGCTCGAGCCCAGTCTGCAGCTGCTGAGCCGCAAGCAGCGGCGACTGATCCGACAGAACCCGGGGATCCT
GCACAGCGTGAGTGGAGGGCTCCAGAGCGCCGTGCGAGAGTGCAAATGGCAATTCCGAAACCGCCGCTGG
AACTGCCCCACTGCTCCGGGGCCCCACCTCTTCGGCAAGATCGTCAACCGAGGCTGCCGAGAAACAGCGT
TCATCTTCGCAATCACCTCCGCCGGGGTCACACATTCCGTGGCGCGCTCCTGCTCCGAAGGCTCCATCGA
GTCCTGCACCTGCGACTACCGGCGGCGCGGCCCTGGGGGCCCCGACTGGCACTGGGGGGGCTGCAGTGAC
AACATCGATTTTGGTCGCCTCTTTGGCCGAGAGTTCGTGGACTCCGGGGAGAAGGGGCGGGACCTACGCT
TCCTCATGAACCTTCACAACAACGAGGCAGGGCGAACGACCGTGTTCTCTGAGATGCGCCAAGAGTGCAA
ATGCCACGGGATGTCCGGCTCCTGCACGGTGCGCACGTGTTGGATGCGGCTGCCCACGCTGCGCGCTGTG
GGCGACGTGCTGCGCGACCGCTTCGACGGTGCCTCCCGCGTCCTTTACGGCAACAGAGGCAGCAACCGCG
CCTCGCGGGCGGAGCTGCTGCGCCTGGAGCCCGAAGACCCCGCGCACAAGCCTCCCTCCCCTCACGACCT
CGTCTACTTCGAGAAATCGCCCAACTTCTGCACGTACAGTGGCCGCCTGGGCACAGCTGGCACAGCTGGA
CGAGCTTGCAACAGCTCGTCTCCCGCGCTGGACGGCTGTGAGCTGCTGTGCTGTGGCCGAGGCCACCGCA
CGCGCACGCAGCGCGTCACGGAGCGCTGCAACTGCACCTTCCACTGGTGCTGCCACGTCAGCTGCCGCAA
CTGCACGCACACGCGCGTTCTGCACGAGTGTCTATGAGGTGCCGCGCCTCCGGGAACGGGAACGCTCTCT
TCCAGTTCTCAGACACACTCACTGGTCCTGATGTTTGCCCACCCTACCGCGTCCAGCCACAGTCCCAGGG
TTCATAGCGATCCATCTCTCCCACCTCCTACCTGGGGACTCCTGAAACCACTTGCCTGAGTCGGCTCGAA
CCCTTTTGCCATCCTGAGGGCCCTGACCCAGCCTACCTCCCTCCCTCTTTGAGGGAGACTCCTTTTGCAC
TGCCCCCCAATTTGGCCAGAGGGTGAGAGAAAGATTCTTCTTCTGGGGTGGGGGTGGGGAGGTCAACTCT
TGAAGGTGTTGCGGTTCCTGATGTATTTTGCGCTGTGACCTCTTTGGGTATTATCACCTTTCCTTGTCTC
TCAGGTCCCTATAGGTCCCTTGAGTTCTCTAACCAGCACCTCTGGGCTTCAAGGCCTTTCCCCTCCCACC
TGTAGCTGAAGAGTTTCCGAGTTGAAAGGGCACGGAAAGCTAAGTGGGAAAGGAGGTTGCTGGACCCAGC
AGCAAAACCCTACATTCTCCTTGTCTCTGCCTCGGAGCCATTGAACAGCTGTGAACCATGCCTCCCTCAG
CCTCCTCCCACCCCTTCCTGTCCTGCCTCCTCATCACTGTGTAAATAATTTGCACCGAAATGTGGCCGCA
GAGCCACGCGTTCGGTTATGTAAATAAAACTATTTATTGTGCTGGGTTCCAGCCTGGGTTGCAGAGACCA
CCCTCACCCCACCTCACTGCTCCTCTGTTCTGCTTGCCAGTCCTTTTGTTATCCGACCTTTTTTCTCTTT
TACCCAGCTTCTCATAGGCGCCCTTGCCCACCGGATCAGTATTTCCTTCCACTGTAGCTATTAGTGGCTC
CTCGCCCCCACCAATGTAGTATCTTCCTCTGAGGAATA
nt 138 - nt 2345 of BC005449.1
Notes:The Wnt1 template used to generate the probe in this study was obtained from A. McMahon. The insert sequence of this clone has been verified by Summerhurst and Murphy (it spans nt 138-2345 of the Wnt1 cDNA sequence BC005449.1). Probe was transcribed from the entire insert.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2006.12.002] [ PMID:17280871] Miller SF, Summerhurst K, Runker AE, Kerjan G, Friedel RH, Chedotal A, Murphy P, Mitchell KJ 2007 Expression of Plxdc2/TEM7R in the developing nervous system of the mouse. Gene Expr Patterns (7):635-44
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE