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EMAGE:10036

1600012H06Rik RIKEN cDNA 1600012H06 gene ( MGI:1915162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10036 EMAGE:10036 EMAGE:10036 EMAGE:10036 EMAGE:10036
"Pseudo-wholemount" of euxassay_008189. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008189_01 euxassay_008189_02 euxassay_008189_03 euxassay_008189_04

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10036Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10036_wholemount_strong.wlz
10036_wholemount_moderate.wlz
10036_wholemount_weak.wlz
10036_wholemount_possible.wlz
10036_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10036_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
moderate moderate
spottedmoderate expression: see section 01 02 03 04
pituitary gland
moderate moderate
spottedmoderate expression: see section 02 03 04 weak expression: see section 01
heart ventricle
weak weak
spottedweak expression: see section 02 03 04
tongue
weak weak
regionalweak expression: see section 01 02 03 04
urethra of male
moderate moderate
regionalmoderate expression: see section 01 02 03
lung
moderate moderate
spottedmoderate expression: see section 01 02 03 04
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T374
Entity Detected:1600012H06Rik, RIKEN cDNA 1600012H06 gene ( MGI:1915162)
Sequence:sense strand is shown

>T374
CAGCGACCTAGGAGAAAAATTGTTAGTTAATATAGAGTGGAATTTTATTTTTATAGGACTGAGTACAAAC
CATCTAATCTCATTCCTGAGCTATCTGTAGTGATAACTCCCTGGGATTTTTCAGTGGGAGGGAGTACAGA
CTGGTTCCTTAAGCCAAGGAAGAGAATGTAAACAACCCTTAATCAGTGTAACCAATGCAGTGTGAACGTT
AGTGCATGGCCAAACGGCATTGTAAAAATCATTTGTACCTTAAACAAAACTACTGTTACCCTTCTGAAAA
TTCAAATACCATCATGCTTTTGAAAGCTCAACAACACAATTCCTAATGTAGAACATGTTAAAGAAGCTGG
CTTTCTATGGCCTTTTCCTCCACTATTCTAACAGTGTTCTCTCTCTCTCTCCCTCTCTATCCCTCTCCAT
GCACTTTAAAACTAGCACCTATCCCATACACTTGATCTGAATTACTACAAAAACCTAAGTGA
Notes:The probe template was PCR amplified from IMAGE:3156193 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3156193 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10038 same embryo
 EMAGE:10037 same embryo
 EMAGE:10041 same embryo
 EMAGE:10040 same embryo
 EMAGE:10039 same embryo
 EurExpress:euxassay_008189 same experiment
 MGI:4822611 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS