EMAGE mouse gene expression spatial database

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EMAGE:10134

Pard3 par-3 (partitioning defective 3) homolog (C. elegans) ( MGI:2135608)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000152. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000152_01	euxassay_000152_02	euxassay_000152_03	euxassay_000152_04

euxassay_000152_05	euxassay_000152_06	euxassay_000152_07	euxassay_000152_08	euxassay_000152_09

euxassay_000152_10	euxassay_000152_11	euxassay_000152_12	euxassay_000152_13	euxassay_000152_14

euxassay_000152_15	euxassay_000152_16	euxassay_000152_17	euxassay_000152_18	euxassay_000152_19

euxassay_000152_20	euxassay_000152_21	euxassay_000152_22	euxassay_000152_23	euxassay_000152_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10134_wholemount_strong.wlz

10134_wholemount_moderate.wlz

10134_wholemount_weak.wlz

10134_wholemount_possible.wlz

10134_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10134_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1592
Entity Detected:	Pard3, par-3 (partitioning defective 3) homolog (C. elegans) ( MGI:2135608)
Sequence:	sense strand is shown >T1592 TACCATCAGTGACTCAGCCGACTGCTCATTGAGTCCAGATGTTGATCCGGTTCTCGCTTTTCAACGGGAA GGATTTGGACGCCAGAGTATGTCAGAAAAACGCACAAAGCAATTTTCAGATGCCAGTCAATTGGATTTCG TTAAAACACGAAAATCAAAAAGCATGGATTTAGTAGCTGACGAGACTAAACTCAATACAGTGGATGACCA GAGAGCAGGCTCTCCCAGTAGAGATGTGGGACCCTCCTTAGGTCTGAAGAAATCTAGCTCCTTAGAAAGT CTGCAGACCGCTGTCGCCGAGGTGACGCTGAACGGGAACATTCCTTTCCACCGCCCTCGGCCACGAATCA TCCGGGGAAGGGGCTGCAACGAAAGCTTCAGAGCCGCCATTGACAAGTCCTACGACAAGCCCATGGTTGA TGATGACGACGAAGGCATGGAGACCTTGGAAGAAGACACAGAGGAAAGCTCGAGGTCAGGGAGGGAGTCC GTGTCCACGTCCAGCGATCAGCCTTCCTATTCTCTGGA
Notes:	The probe template was PCR amplified from IMAGE:989690 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:989690 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10130 same embryo
	EMAGE:10131 same embryo
	EMAGE:10132 same embryo
	EMAGE:10133 same embryo
	EurExpress:euxassay_000152 same experiment
	MGI:4827049 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source