EMAGE mouse gene expression spatial database

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EMAGE:10144

P2rx3 purinergic receptor P2X, ligand-gated ion channel, 3 ( MGI:1097160)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000010. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000010_01	euxassay_000010_02	euxassay_000010_03	euxassay_000010_04

euxassay_000010_05	euxassay_000010_06	euxassay_000010_07	euxassay_000010_08	euxassay_000010_09

euxassay_000010_10	euxassay_000010_11	euxassay_000010_12	euxassay_000010_13	euxassay_000010_14

euxassay_000010_15	euxassay_000010_16	euxassay_000010_17	euxassay_000010_18	euxassay_000010_19

euxassay_000010_20	euxassay_000010_21	euxassay_000010_22	euxassay_000010_23	euxassay_000010_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10144_wholemount_strong.wlz

10144_wholemount_moderate.wlz

10144_wholemount_weak.wlz

10144_wholemount_possible.wlz

10144_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10144_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate

strong

regional

strong expression: see section 09 10 11 17 18

facial vii ganglion

strong

homogeneous

strong expression: see section 06 07 08 19 20 21 22 23

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 07 20 21

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23 24

vagus x ganglion

strong

homogeneous

strong expression: see section 08 09 10 19 20

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 07 08 09 18 19 20

dorsal root ganglion

strong

homogeneous

strong expression: see section 06 10 11 12 18 19 20 not examined expression: see section 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1690
Entity Detected:	P2rx3, purinergic receptor P2X, ligand-gated ion channel, 3 ( MGI:1097160)
Sequence:	sense strand is shown >T1690 TGGCCTCGAGCCAGATTCGGCACGAGGCCGAGAACTTCACCATTTTCATCAAGAACAGCATCCGTTTCCC TCTCTTCAACTTTGAGAAGGGAAACCTCCTTCCTAACCTCACCGACAAGGACATAAAGAAGTGCCGCTTC CATCCTGAAAAGGCCCCATTTTGCCCCATCTTGAGGGTAGGGGACGTGGTTAAGTTTGCTGGGCAGGATT TTGCCAAGCTGGCCCGCACGGGTGGAGTTCTGGGCATTAAGATCGGCTGGGTGTGTGATCTGGACAAGGC CTGGGACCAGTGCATTCCCAAATATTCCTTCACTCGGCTAGATGGAGTTTCTGAGAAAAGCAGTGTTTCC CCTGGCTACAACTTCAGGTTTGCCAAATACTACAAGATGGAGAATGGCAGCGAGTACCGCACGCTCCTGA AGGCTTTCGGCATCCGCTTTGATGTGCTGGTATACGGGAACGCTGGCAAGTTCAACATCATTCCCACCAT CATCAGCTCGGTGGCTGCCTTCACTTCCGTGGGAGTGGGGACTGTTCTCTGTGACATCATCCTG
Notes:	The probe template was PCR amplified from IMAGE:476341 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:476341 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10141 same embryo
	EMAGE:10140 same embryo
	EMAGE:10143 same embryo
	EMAGE:10142 same embryo
	EMAGE:10145 same embryo
	EurExpress:euxassay_000010 same experiment
	MGI:4827013 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source