EMAGE mouse gene expression spatial database

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EMAGE:10339

Ephb3 Eph receptor B3 ( MGI:104770)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018944. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018944_01	euxassay_018944_02	euxassay_018944_03	euxassay_018944_04

euxassay_018944_05	euxassay_018944_06	euxassay_018944_07	euxassay_018944_08	euxassay_018944_09

euxassay_018944_10	euxassay_018944_11	euxassay_018944_12	euxassay_018944_13	euxassay_018944_14

euxassay_018944_15	euxassay_018944_16	euxassay_018944_17	euxassay_018944_18	euxassay_018944_19

euxassay_018944_20	euxassay_018944_21	euxassay_018944_22	euxassay_018944_23	euxassay_018944_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10339_wholemount_strong.wlz

10339_wholemount_moderate.wlz

10339_wholemount_weak.wlz

10339_wholemount_possible.wlz

10339_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10339_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 20 21 22 23 weak expression: see section 10 18 19

vagus x ganglion

weak

regional

weak expression: see section 10 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 weak expression: see section 20

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 18 19 20 weak expression: see section 16 17

neural retina

weak

regional

weak expression: see section 01 02 03 04 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50689
Entity Detected:	Ephb3, Eph receptor B3 ( MGI:104770)
Sequence:	sense strand is shown >T50689 CCAGGACTGCACAAGGATTCTGACCAGCCAGCTGGACTTTTGGATGCCTGGCCTCTGGCTGTGGCCCAGA AGATGGAAGTTTGGGGGAGGACCCTAGCTGTGACTTCTCCAGGCCTGTGCTCCCTCCCAGGAAGTGTGCC CCAAACCTCTTCATATTGAAGATGGATTAGAAGAGGGGGTGATGTCCCCTCCCCAGATGCCTTGGGGCCC AGGCCTGCCTGCTCTCCAGTGGGGAGTCTTCACAACTCAGATTGGGCTGCGCTTCAGTAATGGACGTCCT GGTAGGGTCAGGTGGGGATAAGCCTGGGTTCTTCAGGCCCCAGCCCTGGCAGGGGTCTGACCCCACCAGG TAAGCAGAGAGTACTCCCTCCCCCAGGAAGTGGAGGAGGAGACTCTGGGGATGGGGAGATATGGTGCCCC ATCCTGAAGCCAGCTGGTACCTCCAGTTTGCACAGGGACTTGATGGGGGCTGAGGGCCCTGCCTACCCTT GGTGCTGTCATAAAAGGGCAGGCAGGAGCAGGCTGAGAAGCAGCCTGTGCCTCCCAGAGACTGACTCAGA GAGCCAGAGACTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAAAGACGGGGG TGGGGGTATGTATGTGTGTGTTGTGCACATGCTTGCCTGCACAGAGAGCATGAGTGTGTACAAGCTTGGC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCAGGACTGCACAAGGATTCTGAC; Reverse Primer - name:unspecified, sequence:GCCAAGCTTGTACACACTCATGCT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10340 same embryo
	EMAGE:10342 same embryo
	EMAGE:10343 same embryo
	EMAGE:10344 same embryo
	EMAGE:10341 same embryo
	EurExpress:euxassay_018944 same experiment
	MGI:4824591 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source