EMAGE mouse gene expression spatial database

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EMAGE:10341

Ephb2 Eph receptor B2 ( MGI:99611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018956. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018956_01	euxassay_018956_02	euxassay_018956_03	euxassay_018956_04

euxassay_018956_05	euxassay_018956_06	euxassay_018956_07	euxassay_018956_08	euxassay_018956_09

euxassay_018956_10	euxassay_018956_11	euxassay_018956_12	euxassay_018956_13	euxassay_018956_14

euxassay_018956_15	euxassay_018956_16	euxassay_018956_17	euxassay_018956_18	euxassay_018956_19

euxassay_018956_20	euxassay_018956_21	euxassay_018956_22	euxassay_018956_23	euxassay_018956_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10341_wholemount_strong.wlz

10341_wholemount_moderate.wlz

10341_wholemount_weak.wlz

10341_wholemount_possible.wlz

10341_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10341_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 04 05 06 07 08 22 23 24

foot mesenchyme

weak

regional

weak expression: see section 03 22 23

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 05 06 07 21 22 23 weak expression: see section 08 09 10 11 12 18 19

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 21 22 23 weak expression: see section 08 09 10 11 12 18 19 20

mandible

weak

regional

weak expression: see section 04 05 06 09 10 19 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50372
Entity Detected:	Ephb2, Eph receptor B2 ( MGI:99611)
Sequence:	sense strand is shown >T50372 GTCAAAGGAAGGAAAAGGGGATTGGGTGGGGGGGACACGCACCTAGATCTGGGAGGGGGCGGGACAGGCA AGAGACGTTCGTTCCAAGAGGATTCTCATAAGGGAAGTGACGGTTCTTAGAGGAGAAAAGCAAAAGGCTT GGGAGATCCACATGGCCTGTCCCCGTGAAGGCCAAAAAGCATTTTCTCTCCAACTCCTTCTGGGAAGGTT GAGCTGGCCAGAGCCAAGAAACACTTCCAGGAACATTAACATGAAGGGAAGAGGTGAGCCACCCTCCTTG CCTGGCCTTAGCCGTTTCTTGGAGCATCACTAATGGCCCAGCCCCCAGGCACAAGGGGCAGAGAGACAGG CAGTCGCCCCAGATAGGCTCCGGGGAAGTCCATTCCAGCTGCCACCGGCAGACAGGAGGATAGTTGTTCT GTCTTGGGAGACGGTTTCCAAAAACTCCGAGAGACTTTCTGCTAATGGTTCAGGAGGCGGAAGAGGAGCT CCTGGCCTCCCAGGTCACGCCAAACAGGCTAGATCTTCCTGAGGACAGCCACCCAGGTCTGCAGCCCCAC AAGGCCCTGCTCCTGATCAGCCTCAGCCAGAGGACGAGGAGGCAGGTGACTGTCATCAGCCTCGACTGCC ACAGAGAAGCATACTCTTGCATCTGGGTTTGTTTACAGCGATTCCGTGGACCGGGGGTATTTTGATCAGG GGTGGTTTTGGTCTGGGAGGGTTTTGTTTGTTGGGTTTTTTTAAAAAAAAATGACAATGAAGAGACACTT TGACATTTCCTACCTTTTGAGGACTTGATCCTTCTCCAGG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GTCAAAGGAAGGAAAAGGGGATTG; Reverse Primer - name:unspecified, sequence:CCTGGAGAAGGATCAAGTCCTCAA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10339 same embryo
	EMAGE:10340 same embryo
	EMAGE:10342 same embryo
	EMAGE:10343 same embryo
	EMAGE:10344 same embryo
	EurExpress:euxassay_018956 same experiment
	MGI:4824590 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source