EMAGE mouse gene expression spatial database

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EMAGE:10342

Ephb6 Eph receptor B6 ( MGI:1096338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018946. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018946_01	euxassay_018946_02	euxassay_018946_03	euxassay_018946_04

euxassay_018946_05	euxassay_018946_06	euxassay_018946_07	euxassay_018946_08	euxassay_018946_09

euxassay_018946_10	euxassay_018946_11	euxassay_018946_12	euxassay_018946_13	euxassay_018946_14

euxassay_018946_15	euxassay_018946_16	euxassay_018946_17	euxassay_018946_18	euxassay_018946_19

euxassay_018946_20	euxassay_018946_21	euxassay_018946_22	euxassay_018946_23	euxassay_018946_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10342_wholemount_strong.wlz

10342_wholemount_moderate.wlz

10342_wholemount_weak.wlz

10342_wholemount_possible.wlz

10342_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10342_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 04 05 22 23 weak expression: see section 06 07 20 21

thalamus mantle layer

weak

regional

weak expression: see section 09 10 15 16

lower jaw incisor

weak

regional

weak expression: see section 11 16

lower jaw molar

weak

regional

weak expression: see section 07 08 19 20

upper jaw incisor

weak

regional

weak expression: see section 11 16

upper jaw molar

weak

regional

weak expression: see section 07 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50375
Entity Detected:	Ephb6, Eph receptor B6 ( MGI:1096338)
Sequence:	sense strand is shown >T50375 TCTGCTCTTCAATGTTGTGTGCAAGGAGTGTGGAGGTCACGGAGAGCCCAGCAGTGGGGGGATGTGTCGC CGCTGCAGGGACGAGGTGCATTTCGACCCCCGCCAGAGGGGACTGACTGAGAGTCGAGTGTTAGTTGGGG GGCTCCGAGCACACGTGCCATACATCTTGGAAGTGCAGGCTGTCAATGGGGTGTCTGAGCTCAGCCCTGA CCCTCCCCAGGCAGCAGCCATCAATGTCAGCACCAGCCACGAAGTCCCTTCTGCAGTCCCTGTGATGCAC CAGGTGAGCCGGGCAGCCAACAGCATCACAGTATCCTGGCCACAGCCTGAGCAAACAAATGGGAACATCC TCGACTACCAGCTGCGCTACTATGACCAGGCAGAAGATGAATCCTACTCCTTCACCATGACCAGTGAGAC CAACACTGCCACTGTGACACGGTTGAGCCCTGGCCACATCTATGGCTTCCAGGTGCGGGCACGGACTGCA GCTGGCCACGGCCCCTATGGGGGCAAAGTCTATTTTCAGACATTGCCTCAAGGTGAGCTGTCCTCCCAGC TTCCAGAGAAGCTGTCCTTGGTGATTGGTTCCATCCTGGGGGCCTTGGCCTTCCTTCTACTGGCAGCCAT CACTGTACTGGCTGTCATCTTCCAGCGGAAGCGCCGTGGGACCGGCTACACAGAACAACTGCAACAATAC AGCAGCCCAGGACTTGGAGTGAAGTATTACATTGACCCTTCTACATACGATGACCCCTGC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCTGCTCTTCAATGTTGTGTGCAA; Reverse Primer - name:unspecified, sequence:GCAGGGGTCATCGTATGTAGAAGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10339 same embryo
	EMAGE:10340 same embryo
	EMAGE:10343 same embryo
	EMAGE:10344 same embryo
	EMAGE:10341 same embryo
	EurExpress:euxassay_018946 same experiment
	MGI:4824594 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source