EMAGE mouse gene expression spatial database

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EMAGE:10555

Slc14a2 solute carrier family 14 (urea transporter), member 2 ( MGI:1351653)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018972. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018972_01	euxassay_018972_02	euxassay_018972_03	euxassay_018972_04

euxassay_018972_05	euxassay_018972_06	euxassay_018972_07	euxassay_018972_08	euxassay_018972_09

euxassay_018972_10	euxassay_018972_11	euxassay_018972_12	euxassay_018972_13	euxassay_018972_14

euxassay_018972_15	euxassay_018972_16	euxassay_018972_17	euxassay_018972_18	euxassay_018972_19

euxassay_018972_20	euxassay_018972_21	euxassay_018972_22	euxassay_018972_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10555_wholemount_strong.wlz

10555_wholemount_moderate.wlz

10555_wholemount_weak.wlz

10555_wholemount_possible.wlz

10555_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10555_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 weak expression: see section 11 13

telencephalon mantle layer

moderate

regional

moderate expression: see section 05 09 10 17 18 weak expression: see section 06 07 08 14 15 16 19

telencephalon ventricular layer

strong

regional

strong expression: see section 11 12 moderate expression: see section 10 weak expression: see section 13

midbrain ventricular layer

strong

regional

strong expression: see section 12 13 moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T51030
Entity Detected:	Slc14a2, solute carrier family 14 (urea transporter), member 2 ( MGI:1351653)
Sequence:	sense strand is shown >T51030 ACTGTAGTTCAGAGTAGCTGAAGAGGAAGAAAGACACAGCCGGCCAGGACAGCAGACATTGGGACACCCA CTTGTCTTCTAGGCTGGCTCTGTTTTCTGCCTTGAATACTCAAGGAAGACTGCAAGATCATATTTAAGGT GATCTCTGCAACAACATCAGCTTCACAAAGTTATCACTGCAAAGTTTGGGACATGGGAGATCTTTCCTGT TTGCTTCCTGAGTGTTTCCAGGTGTGAGTGCATTGCATTGACATCTTCCTGCCTCTGTTGACAAGAAGGA CAAAGCTTGAAAGGCCCTCCATCTACTTCATCAACCGGACCTGAGTGACTGGCTATTTCTTACCACTTGA AGTCATCTGAATAGAAGTTTTCAAGACCACCAAGACCAAGGAAACTTCACATGACAAATGAGTGACCACC ACCCCCTGAAGGAGATGTCTGACAGCAACAGCAGCCCTCTCTTGCCAGAGCCTCTTTCCAGCAGATACAA ATTGTATGAGTCAGAGCTAAGCAGCCCTACCTGGCCTTCAAGCTCCCAGGATACACACCCAGCCCTTCCC CTGCTGGAAATGCCGGAAGAAAAGGATCTCCGCTCATCAGATGAAGACAGCCACATAGTGAAGATTGAAA AACCCAATGAGAGGAATAAAAGGAGAGAAAGTGAGGTGTCCCGCCGGGCTTCTGCGGGCCGGGGAGGCTT CAGCCTCTTCCAAGCCGTGAGCTATCTCACTGGCGACATGAAGGAATGCAAGAACTGGCTGAAAGATAAG CCCCTGGTCCTACAGTTCCTAGACTGGGTCCTTCGGGGAGCAGCTCAGGTGATGTTTGTCAACAACCCCA TTAGTGGGCTCATCATCTTCATAGGGCTCCTAATCCAGAATCCCTGGTGGACGATTGCTGGGACACTGGG GACAGTGGCTTCAACCTTGGCTGCCCTTGCCTTAAGCCAGGACAGGTCAGCCATCGCCTCCGGACTCCAT GGCTACAACGGGATGCTGGTGGGACTGCTGATGGCTGTCTTCTCGGAGAAGTTAGACTACTACTGGTGGC TTCTGTTTCCTGTGACCTTCACATCCATGGCCTGCCCCATTATTTCTAGTGCCTTGAGTACCATCTTCGC CAAGTGGGACCTGCCCGTCTTCACACTGCCCTTCAACATTGCCTTAACACTGTACCTGGCAGCTACGGGC CACTACAACCTTTTCTTCCCCACCACGCTCATCAAGCCTGCGTCTGCAGCTCCCAATATCACCTGGACAG AGATTGAAATGCCTTTGCTGTTACAAACCATCCCTGTTGGGGTCGGCCAGGTCTACGGCTGTGACAACCC CTGGACAGGTGGCATGATCCTGGTGGCTCTGTTTATCTCCTCCCCACTCATCTGCTTACATGCAGCCATA GGGTCAATCGTGGGGCTGCTAGCAGCATTGACTGTGGCCACTCCCTTTGAGACGATCTACCTGGGCCTTT GGAGCTACAACTGTGTCCTCTCCTGCATTGCCATTGGCGGCATGTTCTACGCACTCACCTGGCAGACACA CCTGCTGGCACTTGTCTGTGCCCTGTTCTGTGCATACATGGGAGCTGCCCTCTCCAATACAATGGCAGTG GTTGGCGTGCCCTCAGGCACCTGGGCCTTCTGTCTCTCCACCCTCACCTTCCTCCTCCTCACAAGCAACA ACACTGGCATTTACAAGCTCCCACTCAGCAAAGTCACCTACCCGGAGGCCAACCGCATCTACTTCCTGAC AGTGAGACGCAGTGAAGAGGAGAAGTCCCCCAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10552 same embryo
	EMAGE:10554 same embryo
	EMAGE:10556 same embryo
	EMAGE:10553 same embryo
	EurExpress:euxassay_018972 same experiment
	MGI:4828103 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source