EMAGE mouse gene expression spatial database

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EMAGE:10570

Katnal1 katanin p60 subunit A-like 1 ( MGI:2387638)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000821. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000821_01	euxassay_000821_02	euxassay_000821_03	euxassay_000821_04

euxassay_000821_05	euxassay_000821_06	euxassay_000821_07	euxassay_000821_08	euxassay_000821_09

euxassay_000821_10	euxassay_000821_11	euxassay_000821_12	euxassay_000821_13	euxassay_000821_14

euxassay_000821_15	euxassay_000821_16	euxassay_000821_18	euxassay_000821_19	euxassay_000821_20

euxassay_000821_21	euxassay_000821_22	euxassay_000821_23	euxassay_000821_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10570_wholemount_strong.wlz

10570_wholemount_moderate.wlz

10570_wholemount_weak.wlz

10570_wholemount_possible.wlz

10570_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10570_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3907
Entity Detected:	Katnal1, katanin p60 subunit A-like 1 ( MGI:2387638)
Sequence:	sense strand is shown >T3907 CCACGCGTCCGGGGAGCTACTCAGATGGTGTTAAAGTGACTCTTTGTCTGCAGGGGGCTCCGGGGTGGTC GCTGGATTGGGCGCTGTGCGTCGGGCGGGGGTAGCGCAGGTGTCTGAAAGAAGATGAATTTGGCGGAGAT TTGTGAGAATGCGAAGAAAGGCCGGGAATATGCGCTTCTGGGAAATTATGACTCGTCAATGGTGTATTAC CAGGGAGTGATACAGCAGATCCAGAGACACTGCCAGTCACTGAGAGACCCGGCCACCAAAGCCAAGTGGC AGCAGGTACGGCAGGAACTCTTGGAAGAATATGAACAGGTTAAGAGTATCGTCAGCACTTTAGAAAGCTT TAAGATGGACAAGCCCCCTGACTTCCCCGTGTCTTGCCGAGATGAACCGTTTAGAGACCCTGCAGTATGG CCACCCCCTGTCCCTGCGGAACACAGAGCACCCCCTCAAATCAGGCGTCCAAATCGCGAAGTGAGGCCTC TGCGGAAAGACGTGGGGGCAGGAGCCCGGGGACTCGTGGGCCGAGCACACCAGATATCGAAGAGTGACAA ACCTGCAAGTCGGGACAAGGACTATAGAGCAAGAGGGAGAGATGACAAGGCAAGGAAAAATGTGCAAGAT GGTGCAAGTGACAGCGAGATTCCCAAGTTTGATGGCGCCGGGTATGATAAGGATCTGGTGGAAGCCCTGG AGAGGGACATTGTGTCCAGGAACCCTAGCATTCACTGGGATGACATAGCAGACCTGGAGGAGGCTAAGAA GTTGCTCCGGGAAGCTGTTGTCCTGCCCATGTGGATGCCTGACTTTTTCAAAGGGATTAGAAGGCCATGG AAGGGTGTGCTGATGGTTGGCCCCCCAGGCACTGGTAAGACTATGCTGGCTAAAGCGGTTGCCACTGAAT GTGGGACAACCTTTTTCAACGTGTCCTCTTCTACCCTGACATCTAAGTACAGAGGCGAATCTGAGAAGTT GGTCCGACTGTTGTTTGAAATGGCTAGGTTCTATGCCCCTACCACGATCTTCATCGACGAAATTGATTCT ATCTGCAGTCGAAGAGGGACGTCTGATGAGCACGAGGCAAGCCGCAGAGTCAAGTCGGAGCTCCTCATCC AGATGGATGGAGTTGGAGGAGCCTTAGAAAATGATGACCCATCCAAAATGGTGATGGTCCTGGCTGCTAC GAACTTTCCGTGGGACATTGATGAGGCTCTGCGCAGGAGACTAGAGAAAAGGATTTACATTCCTCTCCCG ACAGCAAAAGGAAGAGCGGAGCTCCTGAAGATCAGCCTCCGGGAGGTAGAGCTGGACCCCGATGTCCACC TGGAGGACATCGCCGACAAGACGGAGGGCTACTCGGGTGCCGACATCACTAACATCTGCAGGGACGCTTC TTTGATGGCGATGAGGCGGCGCATCAATGGCTTGAGTCCGGAAGAGATCCGGGCCCTGTCCAAGGAGGAG CTGCAGATGCCTGTCACCAGAGGGGACTTGGAGTTGGCTCTGAAGAAAATCGCCAAGTCTGTCTCAGCGG CAGACCTGGAGAAGTACGAAAAGTGGATGGTTGAGTTTGGGTCTGCATGATTGGTCAGCTCTTCCATCTC TGGGAGTTTTCTTTATGAAATGTGAAGAAATTCCTGAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5369313 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5369313 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10571 same embryo
	EMAGE:10568 same embryo
	EMAGE:10569 same embryo
	EurExpress:euxassay_000821 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source