EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10599

Tbx15 T-box 15 ( MGI:1277234)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000953. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000953_01	euxassay_000953_02	euxassay_000953_03	euxassay_000953_04

euxassay_000953_05	euxassay_000953_06	euxassay_000953_07	euxassay_000953_08	euxassay_000953_09

euxassay_000953_10	euxassay_000953_11	euxassay_000953_12	euxassay_000953_13	euxassay_000953_14

euxassay_000953_15	euxassay_000953_16	euxassay_000953_17	euxassay_000953_18	euxassay_000953_19

euxassay_000953_20	euxassay_000953_21	euxassay_000953_22	euxassay_000953_23	euxassay_000953_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10599_wholemount_strong.wlz

10599_wholemount_moderate.wlz

10599_wholemount_weak.wlz

10599_wholemount_possible.wlz

10599_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10599_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 05 06 21 moderate expression: see section 03 04 07 08 19 20 weak expression: see section 09 10 11

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

dermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 19 20 21 22 23 24

not examined

regional

not examined expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T415
Entity Detected:	Tbx15, T-box 15 ( MGI:1277234)
Sequence:	sense strand is shown >T415 CTGACCTTTGAGGACTTCACTACCATGCAAAAGCAGCAAGGGGGCAGTACAGGTACTTCTCCAACCACCT CCAGCACTGGGACACCATCCCCTTCGGCTTCTTCTCATCTCTTATCTCCATCTTGTTCTCCTCCAACTTT TCATTTGGCCCCGAATACTTTCAATGTGGGCTGTCGAGAAAGCCAGCTGTGTAATCTAAACCTCTCTGAT TATCCACCATGTGCCCGAAGCAACATGGCTGCCTTACAGAGCTATCCAGGGCTGAGTGACAGTGGCTACA ACCGGCTCCAGAGTGGCACAGCTTCAGCCACTCAGCCCTCTGAAACCTTCATGCCTCAGAGGACTCCATC TCTGATCTCAGGAATACCAACACCTCCCTCGTTGCCCAGCAACAGCAAGATGGAAGCCTATGGTGGCCAG TTGGGGTCCTTCCCCACTTCCCAGTTTCAGTATGTCATGCAGGCAGGCAATGCAGCCTCCAGTTCCTCAT CGCCACACATG
Notes:	The probe template was PCR amplified from IMAGE:3157744 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3157744 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10600 same embryo
	EMAGE:10601 same embryo
	EurExpress:euxassay_000953 same experiment
	MGI:4828621 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source