EMAGE mouse gene expression spatial database

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EMAGE:10601

Osbpl3 oxysterol binding protein-like 3 ( MGI:1918970)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000946. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000946_01	euxassay_000946_02	euxassay_000946_03	euxassay_000946_04

euxassay_000946_05	euxassay_000946_06	euxassay_000946_07	euxassay_000946_08	euxassay_000946_09

euxassay_000946_10	euxassay_000946_11	euxassay_000946_12	euxassay_000946_13	euxassay_000946_14

euxassay_000946_15	euxassay_000946_16	euxassay_000946_17	euxassay_000946_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10601_wholemount_strong.wlz

10601_wholemount_moderate.wlz

10601_wholemount_weak.wlz

10601_wholemount_possible.wlz

10601_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10601_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

thalamus mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13

medulla oblongata basal plate

moderate

homogeneous

moderate expression: see section 07

medulla oblongata basal plate mantle layer

moderate

homogeneous

moderate expression: see section 08 09

medulla oblongata basal plate ventricular layer

moderate

homogeneous

moderate expression: see section 10

cerebellum intraventricular portion ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05

pons mantle layer

moderate

homogeneous

moderate expression: see section 08 09

pons ventricular layer

moderate

homogeneous

moderate expression: see section 10

midbrain mantle layer

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13

midbrain ventricular layer

weak

regional

weak expression: see section 10

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 05 weak expression: see section 12 13

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 05 weak expression: see section 12 13

trigeminal v ganglion

strong

homogeneous

strong expression: see section 05 06 07 08 12 13 14 15 16 17 moderate expression: see section 04 weak expression: see section 18

ventral grey horn

weak

regional

weak expression: see section 07 08 09 10

dorsal root ganglion

strong

regional

strong expression: see section 07 moderate expression: see section 05 08 09 10 11 12 13 weak expression: see section 06

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 14

stomach

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10

laryngeal cartilage

moderate

regional

moderate expression: see section 09 10 11 12 13

not examined

regional

not examined expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T238
Entity Detected:	Osbpl3, oxysterol binding protein-like 3 ( MGI:1918970)
Sequence:	sense strand is shown >T238 CCCGGAAGCAATTTGTCATTTTCTTGTGGTGGCGACACTCGAGTTCCTTTCTGGCTACAGTCTTCAGAGG ACATGGAAAAATGTTCAAAAGACCTGGCACACTGCCATGCCTACCTGCTGGAAATGAGCCAGCTCTTAGA AAGCATGGACGTCCTGCATCGGACATACTCGGCGCCAGCCATCAACGCCATCCAGGTCCCTAAGCCTTTT TCTGGCCCTGTGAGACTGCACTCCTCCAATCCTAACTTGTCAACGCTGGACTTTGGAGAAGAGAAATCTT ACTCGGATGGTTCTGAAGCCTCGTCAGAGTTCTCCAAGATGCAGGAGGATCTGTGCCATGTCGCCCACAA AGTTTACTTCGCTTTAAGGTCGGCTTTCAATAGCATATCGGTGGAGAGAGAGAAACTGAAGCAGCTGATG GAGCTGGACACCTCCCCCTCCCCCTCCGCTCAGGTCGTCGGGCTGAAGCACGCTCTGTCATCCGCCCTAG CACAAAACACA
Notes:	The probe template was PCR amplified from IMAGE:2650981 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2650981 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10600 same embryo
	EMAGE:10599 same embryo
	EurExpress:euxassay_000946 same experiment
	MGI:4826989 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source