EMAGE mouse gene expression spatial database

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EMAGE:10645

Echdc1 enoyl Coenzyme A hydratase domain containing 1 ( MGI:1277169)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003212. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003212_01	euxassay_003212_02	euxassay_003212_03	euxassay_003212_04

euxassay_003212_05	euxassay_003212_06	euxassay_003212_07	euxassay_003212_08	euxassay_003212_09

euxassay_003212_10	euxassay_003212_11	euxassay_003212_12	euxassay_003212_13	euxassay_003212_14

euxassay_003212_15	euxassay_003212_16	euxassay_003212_17	euxassay_003212_18	euxassay_003212_19

euxassay_003212_20	euxassay_003212_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10645_wholemount_strong.wlz

10645_wholemount_moderate.wlz

10645_wholemount_weak.wlz

10645_wholemount_possible.wlz

10645_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10645_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 12 13 14 15 16

vibrissa

weak

regional

weak expression: see section 05 06 07 08 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 16 17

cervical ganglion

moderate

regional

moderate expression: see section 09 10 17

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1109
Entity Detected:	Echdc1, enoyl Coenzyme A hydratase domain containing 1 ( MGI:1277169)
Sequence:	sense strand is shown >T1109 CCTCNAGCCTGTTGGCCTACTGGCTGCACCGCAGCGCAGGCGCCGCCGCGCAGGTGGGCTCGTGAGTTCC TGCAGGTCTCTGGGAAATCCTGAGGCGTTGCAGAGAACGTGGGAAAATCCGCTAAATTGGCAGTTTCAAA GGATGAGAAGGTGTGAGGTCAATTCTAAGCCGATTTCTGAATATTTTGGAATCCCTTGTGAGAACCGAGA AATGGCAAAATGTCTTTTGACTTCATCTCTGTCTGTAAGAACAAAACTCTTACAAACAGGAGTGTCGCTT TATAATACGAGTCATGGCTTCCACGAGGAGGAAGTGAAGAAGATACTGGAGCAGTTTCCTGGTGGATCCA TTGACCTCCTAAAGAAGCAGAACGGCATTGGCATTCTGACGCTAAACAACCCCAATAAAATGAATGCCTT CTCAGGTGTCATGATGCTACAACTTTTGGAAAGGGTAATTGAATTAGAAAATTGGACAGAAGGGAAAGGC CTCATTATCCATGGAGCAAAAAATACTTTCTGCTCAGGATCTGATCTGAATGCTGTGAAGGCACTCTCCA CTCCAGAAAGTGGAGTGG
Notes:	The probe template was PCR amplified from IMAGE:2123700 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2123700 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10644 same embryo
	EMAGE:10643 same embryo
	EMAGE:10646 same embryo
	EMAGE:10647 same embryo
	EurExpress:euxassay_003212 same experiment
	MGI:4824463 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source