EMAGE mouse gene expression spatial database

EMAGE:10684

Kctd11 potassium channel tetramerisation domain containing 11 ( MGI:2448712)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000883. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000883_01	euxassay_000883_02	euxassay_000883_03	euxassay_000883_04

euxassay_000883_05	euxassay_000883_06	euxassay_000883_07	euxassay_000883_08	euxassay_000883_09

euxassay_000883_10	euxassay_000883_11	euxassay_000883_12	euxassay_000883_13	euxassay_000883_14

euxassay_000883_15	euxassay_000883_16	euxassay_000883_17	euxassay_000883_18	euxassay_000883_19

euxassay_000883_20	euxassay_000883_21	euxassay_000883_22	euxassay_000883_23

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

single cell

moderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T201
Entity Detected:	Kctd11, potassium channel tetramerisation domain containing 11 ( MGI:2448712)
Sequence:	sense strand is shown >T201 GAAGGCGGTAACGCCCGAGCCCTGAGACACTGTTCCCAACAGAGTCCCGGGACACTCTCAGCTCTGACTG CAAGAGCATCGCCTCTCCCAAGCCATACAGAACTGCTGGCTACCGGCTCCTATCAGTTGCGCCTCCATCT TTTGGGACACACGCCCCTAACCCTGGCTGGGCCTCTGAACAGGTCCTCGGAACCCGACGTGGACTCTACA ACTACAAAAGCTCAGAAACTCAAACTGATCCTTTCAGCGTCCGACCGGTGCTCACCACCGTCACCTGCTG GTTGAATTTCGGAAACCCACTGTCTGAAGAGTACAGGAGGGAATCGCTGACCACGCACAATCAGAAGCGT CTGGACCAAAAGCTCCCTGATCCTGTCTGGCTGCCGTCTGTTCCTTTCATCGGTTCTTTCATCCTCTCGG GGATTTTTGTGTCCTGAAGCCCCCAACCACCACCAAAGTAGCTTGGGTGAGCTACAAACTTTACT
Notes:	The probe template was PCR amplified from IMAGE:2648499 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2648499 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10687 same embryo
	EMAGE:10686 same embryo
	EMAGE:10685 same embryo
	EMAGE:10683 same embryo
	EMAGE:10682 same embryo
	EurExpress:euxassay_000883 same experiment
	MGI:4825735 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source