EMAGE mouse gene expression spatial database

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EMAGE:10687

Wipi2 WD repeat domain, phosphoinositide interacting 2 ( MGI:1923831)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000832. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000832_01	euxassay_000832_02	euxassay_000832_03	euxassay_000832_04

euxassay_000832_05	euxassay_000832_06	euxassay_000832_07	euxassay_000832_08	euxassay_000832_09

euxassay_000832_10	euxassay_000832_11	euxassay_000832_12	euxassay_000832_13	euxassay_000832_14

euxassay_000832_15	euxassay_000832_16	euxassay_000832_17	euxassay_000832_18	euxassay_000832_19

euxassay_000832_20	euxassay_000832_21	euxassay_000832_22	euxassay_000832_23	euxassay_000832_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10687_wholemount_strong.wlz

10687_wholemount_moderate.wlz

10687_wholemount_weak.wlz

10687_wholemount_possible.wlz

10687_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10687_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 11 19 20 21

vibrissa epidermal component

weak

regional

weak expression: see section 07 08 09 10 22 23 24

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 09 10 11 12 13 17 18 19 20 21 22 23 24 weak expression: see section 08

facial vii ganglion

moderate

homogeneous

moderate expression: see section 05 06 weak expression: see section 22 not examined expression: see section 24

inferior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 08 09 weak expression: see section 20 21

superior glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 09 weak expression: see section 08 20

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 05 06 07 09 10 weak expression: see section 08 11 19 20 21 22 23 24

vagus x ganglion

moderate

homogeneous

moderate expression: see section 10 weak expression: see section 19

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 12 13 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T51
Entity Detected:	Wipi2, WD repeat domain, phosphoinositide interacting 2 ( MGI:1923831)
Sequence:	sense strand is shown >T51 TTTTTTTTTTTTTTAAAGTCACTGAAGTGAAAACTGTATTTTCTCAACAGTATGGATAGGCTATAACTAC AACCTCTGCTTTAAGTCTCTGCCATCCTCTCCCTCAATTCAGGTCAAAGTTCATAGCAGCAATGCTCAAG TCCTCAGCCAGGCAGTGTTGTCTGCTCCTGGGCCAGAGTTCACAGGTCAGGATCAGTCAGTCCGGAGAAT CATGGGAGGATGTTCGCTGTCTTCATCCAGGCGCAGAGCGCTGGCTTCATCCTCTAGGCATGCACCACCC ACAGCACCCAGGNCATCTGTGTAN
Notes:	The probe template was PCR amplified from IMAGE:1080066 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1080066 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10684 same embryo
	EMAGE:10686 same embryo
	EMAGE:10685 same embryo
	EMAGE:10683 same embryo
	EMAGE:10682 same embryo
	EurExpress:euxassay_000832 same experiment
	MGI:4829218 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source