EMAGE mouse gene expression spatial database

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EMAGE:10718

Apof apolipoprotein F ( MGI:104539)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001263. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001263_01	euxassay_001263_02	euxassay_001263_03	euxassay_001263_04

euxassay_001263_05	euxassay_001263_06	euxassay_001263_07	euxassay_001263_08	euxassay_001263_09

euxassay_001263_10	euxassay_001263_11	euxassay_001263_12	euxassay_001263_13	euxassay_001263_14

euxassay_001263_15	euxassay_001263_16	euxassay_001263_17	euxassay_001263_18	euxassay_001263_19

euxassay_001263_20	euxassay_001263_21	euxassay_001263_22	euxassay_001263_23	euxassay_001263_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10718_wholemount_strong.wlz

10718_wholemount_moderate.wlz

10718_wholemount_weak.wlz

10718_wholemount_possible.wlz

10718_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10718_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

homogeneous

strong expression: see section 01 05 09 17 18 21 moderate expression: see section 02 03 04 06 07 08 10 11 12 13 14 15 16 19 20 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4914
Entity Detected:	Apof, apolipoprotein F ( MGI:104539)
Sequence:	sense strand is shown >T4914 GAAACAGGAGCAGGATTGTGGAACTACCGAAGCTACCCAGGGACTCTTACTTCTCAATGGCCGGACTGTA TGGGTGCTCAGCTCCTGACATGCATAGCCTCCGACTCATCCTGATGTCAATCCAGCTGCTTTGCTATCTC CTGCTGTGTCCTGTGGATGCCACTTCACACGGAGAGGCAACCAGTGTCTCTACGGTGCTCCCTCCCTCGA AGTTGGGATACCAGATGCAGACCTCAGACAAGCCCTTGTCCTGCCAGATGCTGCTCCCCAAGTCCCTGCC TGGCTTCACTTACATGCCCCCTGTCTCCAAATTCCTGGTAGGCCTGGCTCTAAGGAATGCTCTGGAGGCA GCTGGCTGCCAGGCTGAGGTCTGGGCTCTTCAGCTTCAGCTTTACCGATTAGGAGGTGTGGAGGCTACAC AAGCCCTCATCCACCATCTCCAGGAGCTCCAGAAGAGTGGACACACAGACCGGGAAGTGTCTGTGGATGC CCTGTCCTCTGCTCTGCAGCTCTTAGCCTGGGAGCAGCCAGGTCCAAAGAGGGCCAAACGTTCGATTTCC AACACAGACTGCGACAATGATCAGGAACAGAGTGTCCACAATGTGGTTGACCTACTACCAGCAGTAGGCA CCTACTACAACCTAGGCACAGCTTTGTATTACGCCATTAAGAACTGCTCTGACAAGGCCAAGGAACGAGG TCGAGATGGGGCCATAGATCTGGGTTATGATCTCTTGATGGCCATGGTAGGCGCGTCAGGGGGCCCAGCT GGTGCGGTGATCACGGCTGCTCTTAAGCCAGCGATGAAGGCTGGGGTTCAGCGGCTGATCCAGTATTATT ATGATGAGAAAGAGGTGACCACCCCTCAGCCAGAGGTGACCACCCATCAGCCAGAGACCGGAAAGGATGC CACCACAGATATCGGTGTTGTGGAAGAAATAGCCATGTCTAATTTTGTGTCAGAAGTTGAAAGTACAACT TCTAACTGGGAGTGGCCTTTATTAAAGAACTATGGTGTCTTGGCGTACAAGAGATAGTAACAAAACAACC AATAAGTGGAAAATTCTGACAAGAGAAGTGTAGATGGATGGATGGGTGGGTGGATGGTTTGGGATACTGT CATCGCTTGCCAGTAATTTCCTTCTTGAAACTTAGATTTTTACACTCTAATGGGGCTGGCTAACTTCCAC CTGAACAAGTGATTATAGAAATTAAAAAGCGTTATCATGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5102555 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5102555 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10719 same embryo
	EMAGE:10715 same embryo
	EMAGE:10717 same embryo
	EMAGE:10720 same embryo
	EMAGE:10716 same embryo
	EurExpress:euxassay_001263 same experiment
	MGI:4823191 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source