EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10720

Decr2 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal ( MGI:1347059)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003134. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003134_01	euxassay_003134_02	euxassay_003134_03	euxassay_003134_04

euxassay_003134_05	euxassay_003134_06	euxassay_003134_07	euxassay_003134_08	euxassay_003134_09

euxassay_003134_10	euxassay_003134_11	euxassay_003134_12	euxassay_003134_13	euxassay_003134_14

euxassay_003134_15	euxassay_003134_16	euxassay_003134_17	euxassay_003134_18	euxassay_003134_19

euxassay_003134_20	euxassay_003134_21	euxassay_003134_22	euxassay_003134_23	euxassay_003134_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10720_wholemount_strong.wlz

10720_wholemount_moderate.wlz

10720_wholemount_weak.wlz

10720_wholemount_possible.wlz

10720_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10720_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4921
Entity Detected:	Decr2, 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal ( MGI:1347059)
Sequence:	sense strand is shown >T4921 CGGACGCGTGGGTCCGGACGCGTGGGTTTGCGTTGGAAAGGTAGATCTGGTTCCTGGTTCCACTGTCCTT GCCATCCCGGACACGCAGCAATCATGGCCCAGCCGCCGCCCGACGTTGAGGGGGATGACTGTCTTCCTGA ATACCACCATCTCTTCTGCCCGGATCTTCTCCAGGACAAAGTGGCCTTTATCACAGGTGGTGGTTCTGGG ATTGGCTTCCGGATCGCAGAGATTTTCATGAGGCATGGCTGCCACACTGTCATCGTCGGCAGGAGTCTGC AGAAAGTGACCACGGCTGCTAAGAAGTTGGTTGCTGCCACCGGAAAGCGGTGCCTCCCTCTGTCTATGGA TGTCCGAGTTCCCCCAGAGGTCATGACTGCTGTGGATCAAGCGCTGCAAGAATTTGGCAAAATCAACATC CTCATTAACTGTGCAGCTGGAAACTTCCTATGCCCTGCCAGTGCTTTGTCTTTCAATGCCTTTAAGACCG TGGTTGACATTGACACCATCGGTACCTTCAATGTGTCTAGTGTGCTTTACAAGAAGTTCTTCCGGGACCA TGGAGGAGTGATTGTGAACATTACTGCCACCCTCAGTATGAGGGGGCAGGTGCTACAGCTACATGCAGGC GCTGCCAAAGCAGCTGTGGATGCTATGACGCGACACTTGGCTGTGGAATGGGGTCCCCAGAATATCCGTG TCAACAGCCTGGCTCCTGGTGCCATCAGTGGCACTGAGGGTTTAAGGCGATTAAGAGGCTCCAATGCCAG TTCGAAATTAAAGCATTTTTCAAATCCTATTCCAAGACTTGGGACCAAGACAGAGATCGCTCACAGCGTG CTGTACCTGGCCAGCCCTCTGGCTTCCTATGTCTCAGGTATCGTGCTGGTGGTTGATGGTGGAAGCTGGA TGACATTCCCCAATGGCATCAAGCAACTGCTAGAGTTTGAATCCTTCTCTGCTAAGCTGTAGGGGAGTCC TCCCGTGGATATCCACCTTCTTCTTCTTTGAAGATAGGATCTCATGTACACTATGTAGCTGAAGATGATT TTACAAAAGAGCCACCCTGCCAATTTTTGCAGTGTTTGAAGATCACACCCAAGGCTTCCAGCAGGTTCAA CCAGCAGAATCAACTGATCTGTACGTCCTCCCCCGGGGATCTTCTGACACATAAACTTCGTACTCAAGAT AGTTGTTTTGAGGAGGCAGTGTTCTTCATGGGGAATGCCTCCTGCCTTGCATAGGTCTATAGTCACACAG GAAGTGTTGAGTCTGACTTGGAAATCCGAGCCCACTCCCCTGCCTCTGGGATCAAGCTCTTTGCAAGCTC TGCCTCTCCTCCGCCCACATCTTGAGTAGGAATAACCCGATCATGCATAGCTGAAACTTGCTTACAGTAT CTGAGGAATTCCTATGGTTTTACCAGAAAAAAAATGCATTTTCTTGTAAAAAAAAATTTTTTTTTTTGAG ACAGGGTTTCTCTGCATAGCCCTGGCTGTCCTGGAACTCTGTAGACCAGGCTGGCCTCGAAATCTGCCTG CCTCTGCCTCCCAAGTGCTGGGATTAAAGGCATTCGCCACCACTGCCCGGGTTTTTTTTTTTTTTGTTTT TTGTTTTTTCTACTATAAAAATAGCTATGAGTTGGAATTTGTAAAAGTTTCTAGAGGGCTGGCAAAATGG TTCAGCAGGTAAGAGCACTGACTGCTCTTCTGAAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGC TCACAACCATCTGTGATAAGATCTGGTGCATCTGAAGTCAGCTACAGTGAACTCATGTATAATAATAAAT AAATCTTTGGGCCAGAGCTAGCAGGATTGAGCGAGCAGAGCAGACCAGAGCGAGCAGAGGTCCTAAAAAT TCAATTCCCAACAACCACATGAAGGCTCACAACCATCTGTACAGCTACAGTGTACTCATATACATAAAAT AAATAAAGAAATCTTTAATAAAAACGTTTCTAGACAGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5123899 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5123899 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10718 same embryo
	EMAGE:10719 same embryo
	EMAGE:10715 same embryo
	EMAGE:10717 same embryo
	EMAGE:10716 same embryo
	EurExpress:euxassay_003134 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source