EMAGE mouse gene expression spatial database

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EMAGE:10722

Slc1a7 solute carrier family 1 (glutamate transporter), member 7 ( MGI:2444087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003213. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003213_01	euxassay_003213_02	euxassay_003213_03	euxassay_003213_04

euxassay_003213_05	euxassay_003213_06	euxassay_003213_07	euxassay_003213_08	euxassay_003213_09

euxassay_003213_10	euxassay_003213_11	euxassay_003213_12	euxassay_003213_13	euxassay_003213_14

euxassay_003213_15	euxassay_003213_16	euxassay_003213_17	euxassay_003213_18	euxassay_003213_19

euxassay_003213_20	euxassay_003213_21	euxassay_003213_22	euxassay_003213_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10722_wholemount_strong.wlz

10722_wholemount_moderate.wlz

10722_wholemount_weak.wlz

10722_wholemount_possible.wlz

10722_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10722_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5367
Entity Detected:	Slc1a7, solute carrier family 1 (glutamate transporter), member 7 ( MGI:2444087)
Sequence:	sense strand is shown >T5367 CCACGCGTCCGGGACGCCCCGACCACGTGGCCTGCTCTAATTTCCCAGGCCCTAATCTGCTCCTAGCTCC TAATGATGCAGCTGGTAATAGGATTGTGGCTTCCCTCTGGACGGCTGCACAGACCATGGCTGCAAGGCTG CTGATAGGTGCCTAGGACTCTGTTCACCTGTAAGCAGAGAGGCTTGTGCCTCCAGCCCAGCCCTGGGCCT CATCTGACAGTGCATGGAATGGTGCCAACAGGCTGGGCTCAGCATGGTGCTCGACGCTGTCCTGGCTCGA GGGCGCACAGTGTGTAAGCACAACGGGCTGCTCATCCTGTCTGTGCTCTCTGTCATCGTGGGCTGCCTCC TTGGCTTCTTCCTCAGGACCCAGCGCCTCTCACCACAGGAGATTAGTTACTTCCAGTTCCCTGGAGAACT CTTGATGCGGATGCTGAAGATGCTGATCCTACCACTGGTGGTCTCCAGCTTGATGTCCGGCCTTGCCTCC CTGGATGCCAAGACCTCCAGCCGCTTGGGGATCCTCACCGTGGCATACTACCTGTGGACTACCTTTCTGG CTGTTGTTGTGGGCATCATCATGGTCTCCATCATCCACCCTGGTGGTGCAGCACAGAAGGAGACAACTGA ACAGAGTGGAAAGCCGGTCATGAGCTCAGCTGATGCCCTCCTGGATCTTGTCCGGAACATGTTCCCAGCC AACCTGGTAGAAGCCACGTTCAAACAGTACCGCACCAAGACCACCCCAGTTATCAAGTCTCCCAGGGGAG CAGCGGAGGAGGCTCCCCGGCGGATCGTCATCTATGGGGTCCAGGAAGACAATGGCTCACGTGTGCAGAA CTTTGCCCTGGATCTGACGCCCCCACCTGAGATTGTCTACAAGTCAGAGCCTGGTACCAGTGATGGCATG AACGTGCTAGGCATTGTCATCTTCTCAGCCACGATGGGTATCATGCTGGGCCGCATGGGTGACAGCGGGA CGCCCCTGGTCAGCTTCTGTCAGTGCCTTAATGAGTCTGTCATGAAGATCGTGGCAGTGGCAGGGTGGTA CTTTCCCTTTGGCATTGTCTTCCTGATCGCTGGCAAGATCTTGGAGATGGATGACCCCAAGGCAGTGGGG AAAAAGCTGGGCTTCTACGCCGTGACTGTGGTTTGTGGGCTGGTGGTCCACGGTCTCCTCATCCTGCCCC TACTGTACTTCCTCATCACCAAGAAGAACCCCATCGTCTTCATCCGAGGCGTCCTCCAGGCTCTGCTCAT TGCGTTGGCCACCTCCTCCAGCTCAGCCACACTGCCCATCACCTTCAAGTGCCTGCTGGAGAACAACCAT ATCGACCGCCGCATCGCTCGCTTCGTGCTGCCTGTGGGCGCCACCATCAACATGGATGGCACCGCGCTCT ACGAGGCCGTGGCTGCCATCTTTATCGCCCAGGTCAACAACTATGAGCTAGACTTTGGACAGATCATCAC TATCAGCATCACGGCCACGGCAGCCAGCATTGGGGCGGCTGGCATCCCCCAGGCGGGGCTCGTCACCATG GTCATCGTGCTCACCTCTGTGGGACTGCCGACCGATGACATCAACCTCATCATAGCTGTAGACTGGGCAC TGGATCGCTTCCGAACCATGATTAATGTGCTGGGTGATGCACTGGCGGCAGGGATCATGGCCCACATCTG CCGGAAGGATTTTGCTCAGGACATGGGCACTGAGAAATTGCTACCCTGTGAGACCAAGCCAGTGACACTG CAGGAGATCGTGGCTGCCCAGCAGAATGGCTGTGTGAAGAGTGTGGCTGAGGCCTCAGAGCTAACCCTGG GCCCCACGTGCCCCCACCACATCCCAGTCCAGGTAGAGCAGGATGAGGATCCAGCCGCTGCCAGCCTAGA CCACTGTACTATTGAGATCAGTGAGCTAGAGACCAACGTGTGAACCTGCAGGGCCGCAGCAGAGCCAAGC CAGACCCTGGGGTTTGGGCTAAGGGGTAGGAGCTAAACTCAAAACACACCTGGCGCAGGCTGGCACATTC CACCCTGACAGGCTAGTCAGTCACTGATGAGAAAGAGTGTCAAAAAAGAGACAACTATGTGCAGAGACCC ATCAGCAACTATGGCTCTTGTGGCCCAGAACCTAGCTGTGACAGTTTCTACTACATGAGCACACAAAGGT ACCTGGTAGGGGTCTTTGTCTATACTTTGTGGAAAAATAGTTTGGGAGCCCGAGAGCTAAAACCACTCCC TGAAAGAACAGTCATAGCCAGGATGCTAATTCAAGTCTTTGGAGTCTCCGCCACTCTGTTCTAATAAACA AGGCAGCCTGCAGGGAACAGTGAAAGAGGCTCTGCCTTCTCCAGGTCCCCACGGCCACAACAGCTTCCAA CTCATGAAGATAGAACAACAGTGTCCCTGTTGCTGCCCCAGCTGTGTCCAGGCCAGGTCAACCCTGCCCC ACCCCACAGCATGTGTGTTGGTTTTTTTAAAGATTTATTTATTTATTTTATGTATGTGAGTACACTGTAG CTATCTTCAGACACACCAGAAGAGGGGGGTCAGATTCCATTACAGATGGTTTGTGAGTCACCATGTGGTT GCTGGGATTTGAACACAGGACCTCTGGAAGAGCAGTCAGTGCTCTTAACCACTGAGCCATCTCTCCAGCC CACAGCATGTGGTTTTAACTCAGTAAAGTCTGGTCCATCCCAGGTCTGAGACCTTCTGCCTTCACACGTT ACAAGCCTGTCCGTTTCTGTCCACCAGACCTCTCATCTAATATGGAGACTGATGTCCACTAGGGCTGGGA AGCCGAGCAGGTACAGCCCAGTCAGCCTTCCATGAGATGGGTGGGCAAGGTCGGCCATAAAGAGAAAGAC CCAGCTTGGGATGCTCCGGCCAAGGGACTAGCAGTACCTTGGGGTCAGAACTCTTTGTGTGGCAAAATGG AACAAGAGTCCCTAGTCCCTTCCATGTTTGCGATAATGTGATAAATAAAAGTCTGTTTTTTTTTCAAGAC TTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363389 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363389 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10725 same embryo
	EMAGE:10724 same embryo
	EMAGE:10723 same embryo
	EMAGE:10721 same embryo
	EurExpress:euxassay_003213 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source