EMAGE mouse gene expression spatial database

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EMAGE:10742

Gnb3 guanine nucleotide binding protein (G protein), beta 3 ( MGI:95785)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002925. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002925_01	euxassay_002925_02	euxassay_002925_03	euxassay_002925_04

euxassay_002925_05	euxassay_002925_06	euxassay_002925_07	euxassay_002925_08	euxassay_002925_09

euxassay_002925_10	euxassay_002925_11	euxassay_002925_12	euxassay_002925_13	euxassay_002925_14

euxassay_002925_15	euxassay_002925_16	euxassay_002925_17	euxassay_002925_18	euxassay_002925_19

euxassay_002925_20	euxassay_002925_21	euxassay_002925_22	euxassay_002925_23	euxassay_002925_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10742_wholemount_strong.wlz

10742_wholemount_moderate.wlz

10742_wholemount_weak.wlz

10742_wholemount_possible.wlz

10742_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10742_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 04 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 17

cervical ganglion

moderate

regional

moderate expression: see section 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4328
Entity Detected:	Gnb3, guanine nucleotide binding protein (G protein), beta 3 ( MGI:95785)
Sequence:	sense strand is shown >T4328 GACCGTGGAAGCACCCCTCTATCCCCACCCCCCTTCTTGTGGCTGCATAAGAGGGGTGCTGCTCTTCTCT GGTCCACGGGCCTGTTTCCCACCCCTCCAGGCTGACCTGTCTCTGGAAGCCACATACCTCCCCTCCCTGG CCTCTCCCCCACCCCTGCCAGCTGATTTCATTTGCCTGGCGTGGTTGTTGCTGGCCTTACCCTTCCCTGT CATTCCCCCCTCCCTCTCAGTCAGGGCCAGGCCAGGCCAGCTCCTCTGGCAGCAGAGGGGGGCAGGTGAC AGGCAGGCATCGCAGCTGAGACAGTGAGGAGGCGCCCGGGAAGCGGAAACCTGGGAGAAGTCCAGCCAGA GCCCAAGAGCCGGAACTACCCCTCGACCTGTGCACCATGGGGGAGATGGAGCAGCTGAGGCAGGAGGCGG AGCAACTCAAGAAGCAGATTGCTGATGCCAGGAAAGCCTGTGCGGACATCACTCTGGCTGAGCTTGTGTC TGGCCTGGAGGTGGTGGGCCGAGTCCAGATGCGGACACGGAGGACATTAAGGGGACATCTGGCCAAGATC TATGCCATGCACTGGGCCACTGACTCTAAGCTGCTCGTAAGTGCCTCGCAGGATGGGAAGCTGATCGTGT GGGACACTTATACCACCAATAAGGTGCATGCAATCCCGCTGCGTTCCTCCTGGGTCATGACCTGTGCCTA TGCACCATCAGGGAACTTTGTGGCATGTGGGGGGCTGGACAACATGTGTTCAATCTACAACCTCAAATCC CGCGAGGGCAATGTCAAGGTCAGCCGGGAGCTCTCTGCTCACACAGGTTATCTCTCCTGTTGCCGCTTCC TGGATGACAACAACATCGTGACTAGCTCTGGGGACACCACATGTGCCTTGTGGGACATTGAGACAGGACA GCAGAAGACAGTGTTTGTGGGACACACTGGTGACTGCATGAGCCTGGCTGTGTCCCCAGACTACAAACTC TTCATTTCGGGAGCTTGCGATGCTAGCGCGAAGCTCTGGGATGTGAGGGAAGGGACCTGTCGTCAGACTT TCACTGGCCATGAGTCAGACATCAATGCCATCTGTTTCTTTCCCAACGGGGAGGCCATCTGCACTGGCTC AGATGACGCCTCCTGCCGCCTCTTTGACCTGAGGGCAGACCAGGAACTGACTGCCTATTCCCAGGAGAGC ATCATCTGCGGCATCACTTCAGTAGCCTTCTCGCTCAGTGGGCGCCTGCTCTTTGCAGGCTATGATGACT TCAACTGCAATGTCTGGGACTCTCTGAAGTGCGAGCGTGTAGGCATACTCTCTGGCCATGACAACAGAGT CAGCTGCCTGGGGGTCACTGCTGACGGCATGGCTGTGGCCACTGGTTCCTGGGACAGCTTCCTCAAAATC TGGAACTGAGGAGGCTAGAGGAAGAGGTGGGAAGCCACGAAGGTTCTCAGCAGGCTCCTCCTATGCCCCG TCTCCTTAGGGTCAGTCTCCTATACTCCAGGGGCCATTCCTAGTAAACTTCCTTTTAAGAGCAGGTGGGA TTATGGGAGTGTGCCTTTGGAAGCATCAGGGACTTAAGGGCAAGAACTGCCCCATTTCCTGCCATGGCCT CTCCTCTCCACAGCCCTCACAGCCTCTCCCTTAATAAACAAGAATGGACCCCTTCCCCACCCTAGAGTCC TCCTGGGTTACCAGTAGCCTTTGTCCAGATGGGGAATGGCCAGAGTGCTCAGCCCATGACTATGGATGTG GCTCCTAGTCTCCTGGCTTCCTTCCCGCCGCTTTCTCCTTTTCTGCCCTTTTGTTCTCTCTTATTACCTA ATAAAATGTAGCATCCTGGTACCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4511683 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511683 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10739 same embryo
	EMAGE:10740 same embryo
	EMAGE:10743 same embryo
	EMAGE:10741 same embryo
	EMAGE:10744 same embryo
	EurExpress:euxassay_002925 same experiment
	MGI:4825132 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source