EMAGE mouse gene expression spatial database

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EMAGE:10786

Slc13a1 solute carrier family 13 (sodium/sulfate symporters), member 1 ( MGI:1859937)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001117. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001117_01	euxassay_001117_02	euxassay_001117_03	euxassay_001117_04

euxassay_001117_05	euxassay_001117_06	euxassay_001117_07	euxassay_001117_08	euxassay_001117_09

euxassay_001117_10	euxassay_001117_11	euxassay_001117_12	euxassay_001117_13	euxassay_001117_14

euxassay_001117_15	euxassay_001117_16	euxassay_001117_17	euxassay_001117_18	euxassay_001117_19

euxassay_001117_20	euxassay_001117_21	euxassay_001117_22	euxassay_001117_23	euxassay_001117_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10786_wholemount_strong.wlz

10786_wholemount_moderate.wlz

10786_wholemount_weak.wlz

10786_wholemount_possible.wlz

10786_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10786_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

weak

regional

weak expression: see section 01 02 03 04 05 20 21 22 23 24

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate

weak

regional

weak expression: see section 07 08 18 19

pons ventricular layer

moderate

homogeneous

moderate expression: see section 13

midbrain

weak

regional

weak expression: see section 07 18

midbrain ventricular layer

moderate

regional

moderate expression: see section 13

ventral grey horn

weak

regional

weak expression: see section 12 13 15 16 17 18

nasal capsule

weak

regional

weak expression: see section 10 11 12 13 17 18 19 20

esophagus

moderate

regional

moderate expression: see section 15 weak expression: see section 14 16

kidney calyx

moderate

regional

moderate expression: see section 08 09 10 11 19 20 21 22 weak expression: see section 18

chondrocranium

weak

regional

weak expression: see section 01 02 03 04 05 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4834
Entity Detected:	Slc13a1, solute carrier family 13 (sodium/sulfate symporters), member 1 ( MGI:1859937)
Sequence:	sense strand is shown >T4834 GCTCAGGACAATGAAGCTCCTCAATTATGCTTTGGTCTATCGCCGCTTTCTCCTTGTGGTTTTCACTATT TTGGTTTTCTTGCCACTCCCTCTCATCATCCGCACCAAGGAAGCACAATGTGCCTACATCCTCTTTGTTA TTGCCATATTTTGGATCACAGAAGCCTTGCCCCTGTCAATCACAGCTCTACTGCCTGGGTTAATGTTCCC CATGTTTGGGATCATGCGTTCTTCACAGGTGGCTTCTGCTTATTTCAAAGACTTTCACCTTCTGCTAATT GGAGTCATCTGTTTAGCAACATCAATAGAAAAATGGAATTTGCACAAGAGGATCGCTCTGAGGATGGTGA TGATGGTGGGGGTGAATCCAGCCTGGCTGACGTTGGGGTTCATGAGCAGCACTGCCTTCTTATCTATGTG GCTTAGCAACACCTCTACTGCTGCCATGGTGATGCCCATCGTGGAGGCTGTGGCGCAGCAGATCATCAGT GCTGAAGCAGAGGCCGAGGCCACTCAGATGACTTATTTCAATGAATCTGCCGCCCACGGACTGGACATTG ATGAAACTGTTATTGGACAAGAAACAAATGAGAAAAAAGAGAAAACAAAACCAGCTCCAGGAAGCAGTCA TGACAAAGGGAAAGTGTCAAGAAAGATGGAGACAGAAAAGAATGCAGTCACAGGAGCAAAATATCGGTCA AGGAAGGACCACATGATGTGTAAGCTCATGTGTTTATCTGTTGCTTACTCTTCAACCATTGGTGGGCTGA CAACAATCACGGGTACCTCCACCAACCTGATCTTCTCTGAGCATTTCAATACGCGCTACCCTGATTGTCG CTGCCTCAACTTTGGTTCCTGGTTTTTGTTTTCCTTCCCGGTTGCTCTAATTCTTCTACTTTTGTCTTGG ATTTGGCTCCAGTGGCTCTACCTAGGATTCGACTTTAAGATGTTCAAGTGTGGCAAAACCAAAACACTCA AAGAAAAAGCTTGTGCAAAGGTGATCAAACAAGAATATGAAAAACTTGGGCCAATGAGGTATCAAGAAAT CGTGACCTTGGTGATCTTCATTGTAATGGCCTTGCTCTGGTTCAGTCGGGACCCAGGCTTTGTCACTGGT TGGTCGGTCCTGTTTTCAGAGTACCCGGGTTATGTTACAGATTCAACGGTTGCTTTAGTAGCAGGAATCC TTTTCTTTCTAATTCCAGCTAAGAAAGTGACAAAAATGACATCTGCAGGAGAAATTATTGCTTTTGATTA CACTCCTCTGATTACTTGGAAAGAATTCCAGTCATTCATGCCCTGGGACATAGCCATTCTTGTTGGTGGA GGCTTTGCCCTGGCAGATGGCTGTCAGGTATCAGGACTATCTAACTGGATAGGGAGTAAATTATCTCCTC TAGGTTCATTACCAGTTTGGCTAATAATTCTGATATCTTCCTTGATTGTCACATCTTTGACAGAGGTAGC CAGCAACCCAGCTACCATTACCATTCTGTTCCCAATATTATCTCCTTTGGCTGAAGCCATTCAAGTGAAC CCTCTTCAGATTTTGCTGCCTTCCACTCTCTGTACCTCATTTGCATTTCTTCTGCCAGTTGCAAATCCAC CCAATGCTATTGTTTTTTCCTATGGCCACCTGAAAGTCATTGACATGGTTAAAGCTGGACTTGGAGTGAA TATTTTGGGTGTTGCTGTGGTGATGCTAGGCATGTTCACCTGGATCGAGCCGATGTTTAACCTCCACGAG TATCCCTCCTGGGCTTCTAACTTTGTTAATCAGACCATGCCATGACAAACACACAAGAGCTACCAGTTCA TGGTGACTTTAGGACTTGCTAAGAATTGACTGTACAATGTAGCTGTATAGGACTGGCACATGCAAATCAT CGATACAGCTACGGCAATCGCAGAACATGCCTCACACATGCCCCTTGTCAGCATCTTAATACAGAGTCCG TATCTTTTCAAACCCAATCAAAGAGCATCTACCCGCCTTCGTCCAGAAACTAAGGTGGAGACTGCACTGG CAGACCATCTATGTGCTTGCTCTTTCTCCATAAAAATGATGCTTAAGGACCTTCAAAGAAGTTAGGCCAT TCACCGCATCTTAAACTTGGAGTTCAGTGTAACATTGCAAACATCAACTTATTATTTGAAAATATTTTCT TATGAAACTAAAAGCAGAAAATAAAGTGTGAAAGTAAATGAGACACTTGGATAAGTTATTTTCTATAGTG TTTGTCAAGGGAATTTGCTAAAGTCTCAAGCTATGGCTATTCTGTGTAATGTAAAAAGAAGTGTGTATTT ATTTATTGAAAAGTCTAACTGCATTATTACTGAGGTACAGACAGGACAAGTTTAAATTACAATAGCTACA AGTTCCAAAATTCATGAATCCATTTGATAGTAAAAAATTTATAATGATTAATTTCTACTTGTTCAAATAA ACAGGCTAATATTTATGCTTCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065026 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065026 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10785 same embryo
	EMAGE:10787 same embryo
	EMAGE:10783 same embryo
	EMAGE:10788 same embryo
	EMAGE:10784 same embryo
	EurExpress:euxassay_001117 same experiment
	MGI:4828099 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source